Detection of mRNAs and alternatively spliced transcripts of dopamine receptors in rat peripheral sensory and sympathetic ganglia

• Published in: Brain research Vol. 785; no. 1; pp. 129 - 135
• Main Authors: Xie, Guo-Xi, Jones, Keith, Peroutka, Stephen J, Palmer, Pamela Pierce
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 23.02.1998; Amsterdam Elsevier; New York, NY
• Subjects: Alternative Splicing; Animals; Biological and medical sciences; Carrier Proteins - biosynthesis; DNA Primers; Dopamine Plasma Membrane Transport Proteins; Dopamine receptor; Dopamine transporter; Fundamental and applied biological sciences. Psychology; Ganglia, Spinal - cytology; Ganglia, Spinal - metabolism; Male; Membrane Glycoproteins; Membrane Transport Proteins; Messenger RNA; Nerve Tissue Proteins; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ; Polymerase Chain Reaction - methods; Rats; Rats, Sprague-Dawley; Receptors, Dopamine - biosynthesis; Receptors, Dopamine D1 - biosynthesis; Receptors, Dopamine D2 - biosynthesis; Receptors, Dopamine D3; Receptors, Dopamine D4; Receptors, Dopamine D5; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; Sensory ganglia; Superior Cervical Ganglion - cytology; Superior Cervical Ganglion - metabolism; Sympathetic ganglia; Transcription, Genetic; Vertebrates: nervous system and sense organs; Alternative splicing; DAT, dopamine transporter; DEPC, diethyl pyrocarbonate; mRNA, messenger RNA; Dopamine receptor; cDNA, complementary DNA; Sympathetic ganglia; DRG, dorsal root ganglia; Sensory ganglia; Messenger RNA; TM, transmembrane domain; SCG, superior cervical ganglia; Dopamine transporter; PCR, polymerase chain reaction; bp, base pairs; Spinal cord; Rat; Rodentia; Central nervous system; Superior cervical ganglion; Sympathetic nervous system; Vertebrata; Mammalia; Autonomic nervous system; Spinal ganglion; Carrier protein
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(97)01394-2

The presence of mRNAs for dopamine receptor subtypes and dopamine transporter in rat peripheral sensory and sympathetic ganglia was investigated using polymerase chain reaction (PCR) and DNA sequencing. Dopamine D 1, D 2, D 3, D 5 receptor subtype mRNAs and dopamine transporter mRNA were detected in both superior cervical sympathetic ganglia (SCG) and dorsal root ganglia (DRG) in the rat; the expression of D 4 mRNA was only detected in DRG. While two alternatively spliced isoforms of D 2 were detected in both ganglia, the alternative splicing transcripts for D 3 and D 4 were only found in the DRG. These results are useful in further studying the roles of dopamine and the effects of dopaminergic agents in the peripheral nervous system.