Genome Sequencing of Ciboria shiraiana Provides Insights into the Pathogenic Mechanisms of Hypertrophy Sorosis Scleroteniosis

• Published in: Molecular plant-microbe interactions Vol. 34; no. 1; pp. 62 - 74
• Main Authors: Zhu, Panpan, Kou, Min, Liu, Changying, Zhang, Shuai, Lü, Ruihua, Xia, Zhongqiang, Yu, Maode, Zhao, Aichun
• Format: Journal Article
• Language: English
• Published: United States American Phytopathological Society 01.01.2021; The American Phytopathological Society
• Subjects: Ascomycota - genetics; Carbohydrates; Chromosomes; Control methods; Economic impact; Evolutionary genetics; Gene sequencing; Genes; Genome, Fungal - genetics; Genomes; Host range; Hypertrophy; Hypertrophy - microbiology; Metabolites; Molecular modelling; Morus - microbiology; Pathogenicity; Pathogens; Plant Diseases - genetics; Positive selection; Secondary metabolites; Synteny; Ciboria shiraiana; genome; pathogenicity; proteomics; metabolomics; fungal development; genomics; mulberry; mechanisms of pathogenicity; plant cell wall–degrading enzymes
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/MPMI-07-20-0201-R

causes hypertrophy sorosis scleroteniosis in mulberry trees, resulting in huge economic losses, and exploring its pathogenic mechanism at a genomic level is important for developing new control methods. Here, genome sequencing of based on PacBio RSII and Illumina HiSeq 2500 platform as well as manual gap filling was performed. Synteny analysis with revealed 16 putative chromosomes corresponding to 16 chromosomes of . Screening of rapid-evolution genes revealed that 97 and 2.4% of genes had undergone purifying selection and positive selection, respectively. When compared with , fewer secreted effector proteins were found in . The number of genes involved in pathogenicity, including secondary metabolites, carbohydrate active enzymes, and P450s, in the genome was comparable with that of other necrotrophs but higher than that of biotrophs and saprotrophs. The growth-related genes and plant cell-wall-degradation-related genes in were expressed in different developmental and infection stages, and may be potential targets for prevention and control of this pathogen. These results provide new insights into pathogenic mechanisms, especially host range and necrotrophy features, and lay the foundation for further study of the underlying molecular mechanisms.[Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 "No Rights Reserved" license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.