Lectin histochemistry of Kudoa septempunctata genotype ST3-infected muscle of olive flounder (Paralichthys olivaceus)

• Published in: Parasite (Paris) Vol. 23; p. 21
• Main Authors: Kang, Jaeyoun, Park, Changnam, Jang, Yeounghwan, Ahn, Meejung, Shin, Taekyun
• Format: Journal Article
• Language: English
• Published: France EDP Sciences 2016
• Subjects: Animals; Biotinylation; Carbohydrates; Carbohydrates - analysis; DNA, Mitochondrial - analysis; Epithelial Cells - chemistry; Fish; Fish Diseases - diagnosis; Fish Diseases - metabolism; Fish Diseases - parasitology; Fish Diseases - pathology; Flounder - metabolism; Flounder - parasitology; Food Parasitology; Fucose; Galactose; Genotype; Genotypes; Histochemistry; Histology; Host-Parasite Interactions; Hypertrophy; Immunoenzyme Techniques; Kudoa septempunctata; Lectin; Lectins; Localization; Mannose; Membranes; Muscle, Skeletal - chemistry; Muscle, Skeletal - parasitology; Muscle, Skeletal - pathology; Muscles; Myxozoa; Myxozoa - chemistry; Myxozoa - classification; Myxozoa - genetics; N-Acetylgalactosamine; N-Acetylglucosamine; N-glycans; Olive flounder; Paralichthys olivaceus; Parasitic Diseases, Animal - diagnosis; Parasitic Diseases, Animal - metabolism; Parasitic Diseases, Animal - parasitology; Parasitic Diseases, Animal - pathology; Polysaccharides; Residues; Sequence Alignment; Spores; Substrate Specificity; Sugar
• ISSN: 1776-1042; 1252-607X; 1776-1042
• DOI: 10.1051/parasite/2016021

The localization of carbohydrate terminals in Kudoa septempunctata ST3-infected muscle of olive flounder (Paralichthys olivaceus) was investigated using lectin histochemistry to determine the types of carbohydrate sugar residues expressed in Kudoa spores. Twenty-one lectins were examined, i.e., N-acetylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acetylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, Jacalin, PNA, ECL), complex type N-glycans (PHA-E and PHA-L), and fucose (UEA-I). Spores encased by a plasmodial membrane were labeled for the majority of these lectins, with the exception of LCA, PSA, PNA, and PHA-L. Four lectins (RCA 120, BSL-I, DBA, and SJA) belonging to the galactose/N-acetylgalactosamine group, only labeled spores, but not the plasmodial membrane. This is the first confirmation that various sugar residues are present in spores and plasmodial membranes of K. septempunctata ST3. La localisation des terminaux de carbohydrates dans le muscle du cardeau hirame (Paralichthys olivaceus) infecté par Kudoa septempunctata génotype ST3 a été étudiée en utilisant l’histochimie des lectines, afin de déterminer les types de résidus d’hydrate de carbone exprimés dans les spores de Kudoa. Vingt-et-une lectines ont été examinées, à savoir N-acétylglucosamine (s-WGA, WGA, DSL-II, DSL, LEL, STL), mannose (Con A, LCA, PSA), galactose/N-acétylgalactosamine (RCA12, BSL-I, VVA, DBA, SBA, SJA, jacaline, PNA, ECL), glycanes complexes de type N (PHA-E et PHA-l) et fucose (UEA-I). Les spores enveloppées dans une membrane plasmodiale ont été marquées par la majorité de ces lectines, à l’exception de LCA, PSA, PNA et PHA-l. Quatre lectines (RCA 120, BSL-I, DBA et SJA), appartenant au groupe galactose/N-acétylgalactosamine, ont marqué seulement les spores mais pas la membrane plasmodiale. Ceci est la première confirmation que divers résidus de sucre sont présents dans les spores et les membranes plasmodiales de K. septempunctata ST3.