SEPARATION AND DETECTION OF ANTIOXIDANTS IN VITAMIN A OIL OR IN VEGETABLE OIL BY THIN LAYER CHROMATOGRAPHY

Various antioxidants were developed on silica gel G and polyamide thin layer plates using chloroform, methanol, and benzene as the developing solvents. Although silica gel G generally gave a better separation, a polyamide plate developed with methanol was more satisfactory for separating isoamylgall...

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Bibliographic Details
Published inJournal of vitaminology Vol. 12; no. 2; pp. 112 - 116
Main Authors ISHIKAWA, SEIJI, KATSUI, GOICHIRO
Format Journal Article
LanguageEnglish
Published Japan THE VITAMIN SOCIETY OF JAPAN 01.01.1966
Subjects
Antioxidants - analysis
Chromatography, Thin Layer
Oils - analysis
Vitamin A - analysis
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Summary:Various antioxidants were developed on silica gel G and polyamide thin layer plates using chloroform, methanol, and benzene as the developing solvents. Although silica gel G generally gave a better separation, a polyamide plate developed with methanol was more satisfactory for separating isoamylgallate, propylgallate and nordihydroguaiaretic acid. In order to extract these antioxidants from vitamin A oil and vegetable oil, the compounds were extracted with an equal volume of methanol, and directly developed on a silica gel G or polyamide plate with petroleum ether, benzene, chloroform, and methanol respectively. Separation and detection of as little as 0.1μg of butylhydroxyanisol and dibutylhydroxytoluene and 0.5μg of isoamylgallate and nordihydroguaiaretic acid thus became possible without significant inhibition of the contaminants.
ISSN:0022-5398
DOI:10.5925/jnsv1954.12.112