Oxidized low-density lipoprotein induces the production of superoxide by neutrophils

Exposure of guinea pig peritoneal neutrophils to ox-LDL led to the production of superoxide, which was measured by the formation of superoxide-dependent chemiluminescence. The cells exposed to unoxidized LDL, e.g. native LDL, acetyl-LDL, and self-aggregates of LDL showed no production of superoxide....

Full description

Saved in:
Bibliographic Details
Published inFEBS letters Vol. 377; no. 3; pp. 309 - 312
Main Authors Maeba, Ryouta, Maruyama, Akiko, Tarutani, Osamu, Ueta, Nobuo, Shimasaki, Hiroyuki
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 27.12.1995
Subjects
Animals
Atherosclerosis
Chemiluminescence
Cytochalasin B - pharmacology
Dose-Response Relationship, Drug
Guinea Pigs
Lipid peroxidation
Lipoproteins, LDL - pharmacology
Luminescent Measurements
Neutrophil
Neutrophils - drug effects
Neutrophils - metabolism
Opsonin Proteins - pharmacology
Oxidation-Reduction
Oxidized LDL
Peritoneal Cavity - cytology
Superoxide
Superoxides - metabolism
Tetradecanoylphorbol Acetate - pharmacology
Zymosan - pharmacology
Lipid peroxidation
Superoxide
Chemiluminescence
Neutrophil
Oxidized LDL
Atherosclerosis
Online AccessGet full text

Cover

More Information
Summary:Exposure of guinea pig peritoneal neutrophils to ox-LDL led to the production of superoxide, which was measured by the formation of superoxide-dependent chemiluminescence. The cells exposed to unoxidized LDL, e.g. native LDL, acetyl-LDL, and self-aggregates of LDL showed no production of superoxide. The superoxide production was correlated with the levels of oxidative modification of LDL and reached a maximum between 10 and 30 min during incubation, but preincubating the cells with cytochalasin B decreased the superoxide production. These findings indicate that neutrophils rapidly take up ox-LDL by phagocytosis and generate superoxide which may cause superoxide-mediated lipid peroxidation in vivo.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)01336-9