Invasiveness and Intracellular Growth of Multidrug-Resistant Salmonella and Other Pathogens in Caco-2 Cells

• Published in: Journal of food science Vol. 72; no. 2; pp. M72 - M78
• Main Authors: Kim, S.-H, Wei, C.-I
• Format: Journal Article
• Language: English
• Published: Malden, USA Blackwell Publishing Inc 01.03.2007; Institute of Food Technologists; Wiley Subscription Services, Inc
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Bacteria; Bacterial Adhesion - physiology; bacterial infections; Biological and medical sciences; Caco-2 cell; Caco-2 Cells - microbiology; cell invasion; Cells; Colony Count, Microbial; Drug resistance; Drug Resistance, Bacterial - genetics; Drug Resistance, Multiple, Bacterial - genetics; food contamination; Food Contamination - analysis; Food Handling; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; Genotype; ground beef; human health; Humans; intestines; invasiveness; Klebsiella pneumoniae; Listeria monocytogenes; Meat Products - microbiology; microbial genetics; microbial growth; Microbial Sensitivity Tests; multiple drug resistance; phenotype; Pseudomonas aeruginosa; Public health; Salmonella; Salmonella - drug effects; Salmonella - genetics; Salmonella - growth & development; Salmonella - physiology; Salmonella enterica subsp. enterica serovar Agona; Salmonella enterica subsp. enterica serovar Heidelberg; Salmonella Heidelberg; Salmonella typhimurium; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics; Salmonella typhimurium - growth & development; Salmonella typhimurium - physiology; Salmonella Typhimurium DT104; Species Specificity; zoonoses; Salmonella; Digestive system; Growth; Gut; Bacteria; Intracellular; Enterobacteriaceae
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/j.1750-3841.2006.00268.x

The increase of multidrug-resistant pathogens of human and animal origins is a major public health concern. For a better understanding of the health consequences of multidrug-resistant bacteria transmitted from animal products to humans, the host interaction of zoonotic Salmonella isolates along with other pathogenic and commensal bacteria was evaluated using a human intestinal Caco-2 cell system. Multidrug-resistant S. Agona, S. Heidelberg, and S. Typhimurium possessed plasmid-mediated class 1 integrons. The S. Typhimurium DT104 isolate from ground beef showed the well-known genotypic and phenotypic resistance characteristics of the species, and contained the chromosomally located class 1 integron. Among the multidrug-resistant Salmonella isolates, the S. Heidelberg 219 had the highest invasion number at 1.0 x 10⁴ CFU/mL, followed by the S. Typhimurium DT104 isolate at 7.7 x 10³ CFU/mL. Listeria monocytogenes was the best performer among the tested species in invading the Caco-2 cell. Multidrug-resistant opportunistic pathogens Klebsiella pneumoniae and Pseudomonas aeruginosa were also able to invade the cells. The invasion of S. Heidelberg 219, S. Typhimurium DT104, L. monocytogenes, K. pneumoniae, and P. aeruginosa into the Caco-2 cells was not affected even in the presence of commensal E. coli. During the intracellular growth of S. Heidelberg 219, S. Typhimurium DT104, and L. monocytogenes, the bacterial counts increased 2 log cycles in 9 h in the Caco-2 cells. Therefore, these strains could rapidly proliferate after their invasion into the cells.