Molecular characterisation of the vitellogenin gene (AlVg) and its expression after Apolygus lucorum had fed on different hosts
BACKGROUND The polyphagous pest Apolygus lucorum is now the dominant pest of Bacillus thuringiensis (Bt) cotton in China. In this study, the transcriptional and translational profiles of AlVg influenced by different hosts were identified, and then the correlations between AlVg gene or AlVg protein e...
Saved in:
Published in | Pest management science Vol. 72; no. 9; pp. 1743 - 1751 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.09.2016
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | BACKGROUND
The polyphagous pest Apolygus lucorum is now the dominant pest of Bacillus thuringiensis (Bt) cotton in China. In this study, the transcriptional and translational profiles of AlVg influenced by different hosts were identified, and then the correlations between AlVg gene or AlVg protein expression and key population proliferation parameters of A. lucorum were further clarified.
RESULTS
AlVg or AlVg expression can be significantly regulated by different host nutrients (P < 0.05). AlVg or AlVg expression was significantly higher in A. lucorum reared on Bt and conventional cotton than in A. lucorum reared on garland chrysanthemum and broad bean (P < 0.05), but there was no significant difference between AlVg or AlVg expression in A. lucorum reared on Bt and conventional cotton (P > 0.05). In addition, there were significant linear regression correlations between AlVg or AlVg expression and total mortality rate of nymphs, female lifespan, per female fecundity and egg hatching rates (P < 0.05).
CONCLUSION
Our results confirm that AlVg or AlVg is the key parameter affecting female fertility of A. lucorum. AlVg and AlVg expression can be influenced by different host nutrients except for Bt toxin. © 2015 Society of Chemical Industry |
---|---|
Bibliography: | Jiangsu Agriculture Science and Technology Innovation Fund JASTIF - No. CX(14)5020 State Key Laboratory for Biology of Plant Diseases and Insect Pests - No. SKL2012OP03 ArticleID:PS4203 Special Fund for Agro-scientific Research in the Public Interest of China - No. 201103012-04 ark:/67375/WNG-B0D935M3-8 istex:DE23618095F3B7E7251D32DDEE4AE410A63F5F5F Natural Science Foundation of Jiangsu Province - No. BK20130717 National Natural Science Foundation of China - No. 31401753; No. 31301669 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1526-498X 1526-4998 |
DOI: | 10.1002/ps.4203 |