Systemic oxidative stress in children with cystic fibrosis with bacterial infection including Pseudomonas Aeruginosa

• Published in: The clinical respiratory journal Vol. 16; no. 6; pp. 475 - 483
• Main Authors: Bennemann, Gabriela Datsch, Moreira, Emilia Addison Machado, Pereira, Leticia Cristina Radin, Freitas, Maiara Brusco, Oliveira, Diane, Ventura, Julia Carvalho, Parisotto, Eduardo Benedetti, Moreno, Yara Maria Franco, Trindade, Erasmo Benicio Santos Moraes, Barbosa, Eliana, Ludwig Neto, Norberto, Wilhelm Filho, Danilo
• Format: Journal Article
• Language: English
• Published: Copenhagen John Wiley & Sons, Inc 01.06.2022; John Wiley and Sons Inc; Wiley
• Subjects: antioxidants; bacterial infection; Bacterial infections; Bacteriology; Biomarkers; children; Cystic fibrosis; Infections; Original; Oxidative stress; Brazil; United States > US
• ISSN: 1752-6981; 1752-699X
• DOI: 10.1111/crj.13513

Introduction Oxidative stress (OS) occurs in cystic fibrosis (CF). Objective The objective of this work is to evaluate the influence of bacterial infection on biomarkers of OS (catalase [CAT], glutathione peroxidade [GPx], reduced glutathione [GSH]), markers of oxidative damage (protein carbonyls [PC], thiobarbituric acid reactive substances [TBARS]), together with the nutritional status and lung function in children with CF. Methods Cross‐sectional study including CF group (CFG, n = 55) and control group (CG, n = 31), median age: 3.89 and 4.62 years, respectively. CFG was distributed into CFG negative bacteriology (CFGB−, n = 27) or CFG positive bacteriology (CFGB+, n = 28), and CFG negative Pseudomonas aeruginosa (CFGPa−, n = 36) or CFG positive Pseudomonas aeruginosa (CFGPa+, n = 19). Results Compared with CG, CFG (P = .034) and CFGB+ (P = .042) had lower body mass index‐for‐age z‐score; forced expiratory volume in the first second was lower in CFGB+ and CFGPa+ (both P < .001). After adjusting for confounders and compared with CG: CFG showed higher TBARS (P ≤ .001) and PC (P = .048), and lower CAT (P = .004) and GPx (P = .003); the increase in PC levels was observed in CFGB+ (P = .011) and CFGPa+ (P = .001) but not in CFGB− (P = .510) and CFGPa− (P = .460). Conclusions These results indicate a systemic OS in children with CF. The presence of bacterial infection particularly Pseudomonas aeruginosa seems to be determinant to exacerbate the oxidative damage to proteins, in which PC may be a useful biomarker of OS in CF. We evaluate the influence of bacterial infection on biomarkers of oxidative stress (catalase, glutathione peroxidase, reduced glutathione), markers of oxidative damage (protein carbonyls, thiobarbituric acid reactive substances), together with the nutritional status and pulmonary function in children with cystic fibrosis. Our results demonstrate that there is a systemic oxidative stress in children with cystic fibrosis. The presence of bacterial infection particularly Pseudomonas aeruginosa seems to be determinant to exacerbate the oxidative damage to proteins, in which protein carbonyls may be a useful biomarker of oxidative stress in cystic fibrosis.