A general method for gene isolation in tagging approaches: amplification of insertion mutagenised sites (AIMS)

A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T‐DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The te...

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Published inThe Plant journal : for cell and molecular biology Vol. 13; no. 5; pp. 717 - 721
Main Authors Frey, Monika, Stettner, Cornelia, Gierl, Alfons
Format Journal Article
LanguageEnglish
Published Oxford BSL Blackwell Science Ltd 01.03.1998
Blackwell Science
Subjects
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genes. Genome
Molecular and cellular biology
Molecular genetics
Monocotyledones
Zea mays
Gene
Gramineae
Insertion mutation
Angiospermae
Spermatophyta
Method
Molecular cloning
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Summary:A polymerase chain reaction (PCR) based procedure for the isolation of genes in transposon or T‐DNA tagging approaches has been developed. The method can be generally applied and allows the rapid isolation of putative gene sequences even in the presence of high numbers of insertion sequences. The technique has been used successfully for the isolation of the maize Bx1 gene tagged by a Mutator element.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0960-7412
1365-313X
DOI:10.1046/j.1365-313X.1998.00091.x