Isocitrate Lyase Activity in Lactobacillus murinus CNRZ 313

Isocitrate lyase activity in Lactobacillus murinus CNRZ 313 was demonstrated. In cell-free extracts partially purified by ammonium sulphate precipitation, maximum activity was at 37°C, pH 7.9, in the presence of 16mM glutathione and 2.4mM cysteine. The extracts exhibited an apparent Michaelis consta...

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Bibliographic Details
Published inJournal of dairy science Vol. 66; no. 6; pp. 1232 - 1236
Main Authors Albizzatti de Rivadeneira, M.C., Manca de Nadra, M.C., Pesce de Ruiz Holgado, A.A., Oliver, G.
Format Journal Article
LanguageEnglish
Published Champaign Elsevier Inc 01.06.1983
Am Dairy Sci Assoc
American Dairy Science Association
Subjects
isocitrate lyase
Lactobacillus murinus
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Summary:Isocitrate lyase activity in Lactobacillus murinus CNRZ 313 was demonstrated. In cell-free extracts partially purified by ammonium sulphate precipitation, maximum activity was at 37°C, pH 7.9, in the presence of 16mM glutathione and 2.4mM cysteine. The extracts exhibited an apparent Michaelis constant 1.17×10−2 M. Preincubation of the extract for 20 to 60min at 40°C stimulated enzymatic activity.
Bibliography:Q
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ObjectType-Article-1
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content type line 23
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(83)81928-6