Effect of Brazilian propolis on scratching behavior induced by compound 48/80 and histamine in mice

• Published in: International immunopharmacology Vol. 4; no. 10-11; pp. 1431 - 1436
• Main Authors: Shinmei, Yoshifumi, Hossen, Maria Alejandra, Okihara, Kiyoshi, Sugimoto, Hiroyuki, Yamada, Hideo, Kamei, Chiaki
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.2004; Elsevier
• Subjects: Animals; Antineoplastic agents; Behavior, Animal - drug effects; Biological and medical sciences; Brazil; Capillary Permeability - drug effects; Compound 48/80; Dose-Response Relationship, Drug; Drug Interactions; Female; Histamine; Histamine H1 Antagonists - administration & dosage; Histamine H1 Antagonists - pharmacokinetics; Histamine H1 Antagonists - therapeutic use; Histamine release; Histamine Release - drug effects; Mast Cells - drug effects; Mast Cells - metabolism; Medical sciences; Mice; Mice, Inbred ICR; p-Methoxy-N-methylphenethylamine; Pharmacology. Drug treatments; Propolis; Propolis - administration & dosage; Propolis - pharmacokinetics; Propolis - therapeutic use; Pruritus - drug therapy; Pruritus - etiology; Rats; Rats, Wistar; Scratching behavior; Time Factors; Tumors; Vascular permeability; South America; Brazil; America; Compound 48/80; Scratching behavior; Histamine release; Vascular permeability; Propolis; Rodentia; Permeability; Histamine; Vertebrata; Mammalia; Mouse; Animal; Blood vessel; Circulatory system; Behavior
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2004.06.007

We studied the effect of Brazilian propolis on scratching behavior induced by compound 48/80 and histamine in ICR mice. Propolis granular A.P.C dose-related inhibited scratching behavior induced by compound 48/80 and significant inhibition were observed at 1000 mg/kg. However, histamine-induced scratching behavior was not inhibited by propolis granular A.P.C even at 1000 mg/kg. Propolis ethanol extract at 10 μg/ml or more inhibited histamine release from rat mast cells induced by compound 48/80. In addition, it blocked increased vascular permeability induced by compound 48/80. The inhibitory effect of propolis on scratching behavior induced by compound 48/80 was gradually enhanced by repeated administration, and 500 mg/kg propolis granular A.P.C, which caused no effect through single administration, significantly inhibited scratching behavior after repeated administration for 4 weeks. From these findings, it is assumed that the inhibition of scratching behavior induced by propolis occurs through a mast cell-dependent mechanism.