Metabolomic Study of Aging in fa / fa Rats: Multiplatform Urine and Serum Analysis

Zucker fatty ( / ) rats represent a well-established and widely used model of genetic obesity. Because previous metabolomic studies have only been published for young / rats up to 20 weeks of age, which can be considered early maturity in male / rats, the aim of our work was to extend the metabolomi...

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Published inMetabolites Vol. 13; no. 4; p. 552
Main Authors Pelantová, Helena, Tomášová, Petra, Šedivá, Blanka, Neprašová, Barbora, Mráziková, Lucia, Kuneš, Jaroslav, Železná, Blanka, Maletínská, Lenka, Kuzma, Marek
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 12.04.2023
MDPI
Subjects
Age
Aging
Bile acids
Experiments
fa/fa rats
genetic obesity
Insulin resistance
Laboratory animals
LC-MS
Metabolism
Metabolites
Metabolomics
Neurotransmitters
Nicotinamide
NMR
Nuclear magnetic resonance
Obesity
Plasma
Software
Urine
Italy
Czech Republic
Germany
LC-MS
NMR
genetic obesity
metabolomics
fa/fa rats
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Summary:Zucker fatty ( / ) rats represent a well-established and widely used model of genetic obesity. Because previous metabolomic studies have only been published for young / rats up to 20 weeks of age, which can be considered early maturity in male / rats, the aim of our work was to extend the metabolomic characterization to significantly older animals. Therefore, the urinary profiles of obese / rats and their lean controls were monitored using untargeted NMR metabolomics between 12 and 40 weeks of age. At the end of the experiment, the rats were also characterized by NMR and LC-MS serum analysis, which was supplemented by a targeted LC-MS analysis of serum bile acids and neurotransmitters. The urine analysis showed that most of the characteristic differences detected in young obese / rats persisted throughout the experiment, primarily through a decrease in microbial co-metabolite levels, the upregulation of the citrate cycle, and changes in nicotinamide metabolism compared with the age-related controls. The serum of 40-week-old obese rats showed a reduction in several bile acid conjugates and an increase in serotonin. Our study demonstrated that the / model of genetic obesity is stable up to 40 weeks of age and is therefore suitable for long-term experiments.
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ISSN:2218-1989
2218-1989
DOI:10.3390/metabo13040552