One-pot microbial synthesis of 2'-deoxyribonucleoside from glucose, acetaldehyde, and a nucleobase

• Published in: Biotechnology letters Vol. 28; no. 12; pp. 877 - 881
• Main Authors: HORINOUCHI, Nobuyuki, OGAWA, Jun, KAWANO, Takako, SAKAI, Takafumi, SAITO, Kyota, MATSUMOTO, Seiichiro, SASAKI, Mie, MIKAMI, Yoichi, SHIMIZU, Sakayu
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.06.2006; Springer Nature B.V
• Subjects: Acetaldehyde - metabolism; Biological and medical sciences; Biotechnology; Biotechnology - methods; Deoxyribonucleosides - biosynthesis; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Microbiology; Pentosyltransferases - pharmacology; Phosphotransferases - metabolism; Ribosemonophosphates - metabolism; Saccharomyces - enzymology; Saccharomyces cerevisiae; Transformation, Bacterial - genetics; Transformation, Bacterial - physiology; Yeast; Yeasts; Deoxyribose-phosphate aldolase; Yeast; Nucleoside phosphorylase; Phosphopentomutase; Glycosyltransferases; Lyases; Deoxyribonucleoside; Glucose; Acetaldehyde; Fungi; Isomerases; Nucleoside; Ascomycetes; Phosphotransferases; Thallophyta; Enzyme; Transferases; Aldehyde-lyases; Phosphorylase; Carbon-carbon lyases; 2'-deoxyribonucleoside; Intramolecular transferases; Glycolysis; Hexosyltransferases; Microorganism; Deoxyriboaldolase; Saccharomyces cerevisiae
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-006-9019-5

A one-pot enzymatic synthesis of 2'-deoxyribonucleoside from glucose, acetaldehyde, and a nucleobase was established. Glycolysis by baker's yeast (Saccharomyces cerevisiae) generated ATP which was used to produce D: -glyceraldehyde 3-phosphate production from glucose via fructose 1,6-diphosphate. The D: -glyceraldehyde 3-phosphate produced was transformed to 2'-deoxyribonucleoside via 2-deoxyribose 5-phosphate and then 2-deoxyribose 1-phosphate in the presence of acetaldehyde and a nucleobase by deoxyriboaldolase, phosphopentomutase expressed in Escherichia coli, and a commercial nucleoside phosphorylase. About 33 mM 2'-deoxyinosine was produced from 600 mM glucose, 333 mM acetaldehyde and 100 mM adenine in 24 h. 2'-Deoxyinosine was produced from adenine due to the adenosine deaminase activity of E. coli transformants.