Preparation and characterization of cationic curcumin nanoparticles for improvement of cellular uptake

• Published in: Carbohydrate polymers Vol. 90; no. 1; pp. 16 - 22
• Main Authors: Liu, Jinsong, Xu, Lihua, Liu, Chuantong, Zhang, Dafeng, Wang, Siqian, Deng, Zhennan, Lou, Weiwei, Xu, Haihong, Bai, Qing, Ma, Jianfeng
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.09.2012; Elsevier
• Subjects: Antineoplastic agents; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - pharmacokinetics; Antineoplastic Agents - pharmacology; Applied sciences; Biological and medical sciences; Cations - chemistry; Cell Survival - drug effects; Cell uptake; cell viability; Chemotherapy; chitosan; Chitosan - chemistry; Chitosan/PCL; Curcumin; Curcumin - administration & dosage; Curcumin - pharmacokinetics; Curcumin - pharmacology; cytotoxicity; Delayed-Action Preparations - chemistry; drugs; encapsulation; Exact sciences and technology; fluorescence; HeLa Cells; Humans; In vitro cytotoxicity; Medical sciences; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Natural polymers; neoplasm cells; Neoplasms - drug therapy; Pharmacology. Drug treatments; Physicochemistry of polymers; Polyesters - chemistry; spectral analysis; Starch and polysaccharides; zeta potential; Nanoparticles; Cell uptake; Chitosan/PCL; Curcumin; In vitro cytotoxicity; Biological properties; Antineoplastic agent; Nanoparticle; Cytotoxicity; Drug carrier; Nanoencapsulation; Charged particle; Electrokinetic potential; Manufacturing; Polycaprolactone; Release; Polymer blends; Chemical precipitation; Control release polymer; Experimental study; In vitro; Hela cell line; Internalization; Oside polymer; Chitosan; Kinetics
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.04.036

► A simple nano-precipitation was employed to develop cationic curcumin nanoparticle. ► The cationic carrier was non-cytotoxic suitable for further in vivo application. ► The cationic curcumin nanoparticle greatly improve the cellular uptake of curcumin. In the present paper, cationic nanoparticles of curcumin, chitosan and poly(ɛ-caprolactone) were developed by a simple nano-precipitation method. The developed curcumin loaded chitosan/poly(ɛ-caprolactone) (chitosan/PCL) nanoparticle showed almost spherical shape and its diameter was varied between 220nm and 360nm and zeta potential was varied between +30mV and 0mV as a function with pH value. The encapsulation of curcumin into nanoparticles was confirmed by fluorescence spectral analysis. In vitro release study showed the sustained release behavior of curcumin from nanoparticles during the period of 5 days study. In vitro cytotoxicity test revealed the drug concentration dependent on the cell viability against Hela cells and OCM-1 cells after 48h co-incubation. Furthermore, in vitro cell uptake study revealed that the cell uptake of curcumin was greatly enhanced by encapsulated curcumin into cationic chitosan/PCL nanoparticles. Therefore, the developed cationic chitosan/PCL nanoparticles might be a promising candidate for curcumin delivery to cancer cells.