Preparation of silver nanoparticles in the presence of chitosan by electrochemical method

• Published in: Carbohydrate polymers Vol. 89; no. 1; pp. 236 - 244
• Main Authors: Reicha, Fikry M., Sarhan, Afaf, Abdel-Hamid, Maysa I., El-Sherbiny, Ibrahim M.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 05.06.2012; Elsevier
• Subjects: absorption; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - radiation effects; Antibacterial; antibacterial properties; Applied sciences; Bacillus thuringiensis; Bacillus thuringiensis - drug effects; Bacillus thuringiensis - growth & development; bacteria; Chitosan; Chitosan - chemistry; Chitosan - radiation effects; Cross-disciplinary physics: materials science; rheology; Electrochemical; Electrochemical Techniques; electrochemistry; Exact sciences and technology; Materials science; Metal Nanoparticles - chemistry; Metal Nanoparticles - radiation effects; Microscopy, Electron, Transmission; Nanoparticles; Nanopowders; Nanoscale materials and structures: fabrication and characterization; nanosilver; Natural polymers; oxidation; Oxidation-Reduction; Physicochemistry of polymers; Physics; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - growth & development; Silver; Silver - chemistry; Silver - pharmacology; Silver - radiation effects; Spectroscopy, Fourier Transform Infrared; Starch and polysaccharides; Swelling; transmission electron microscopy; ultraviolet radiation; Ultraviolet Rays; ultraviolet-visible spectroscopy; UV-irradiation; X-Ray Diffraction; Nanoparticles; Silver; UV-irradiation; Swelling; Chitosan; Antibacterial; Electrochemical; Biological properties; Electrochemical method; Nanoparticle; Photochemical method; Experimental study; Metal particle; Absorption spectrum; Bactericidal effect; Chemical reduction; Particle size distribution; Nanocomposite; Oxidation; Kinetics; Chemical synthesis; Successive reaction
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.03.002

► Chitosan-Ag nanoparticles were prepared by a new green electrochemical process. ► The applied new technique is quick and cost-effective. ► The nanoparticles showed high purity and uniform particle size distribution. ► The particle size has been controlled via adjusting the current density. ► The nanoparticles demonstrated promising bioactivity for biomedical applications. The present study involves the development of stabilized and densely dispersed chitosan-silver nanoparticles using a green approach based on electrochemical oxidation/complexation process followed by UV irradiation reduction. Formation of the nanoparticles was confirmed by appearance of surface plasmon absorption around 420nm. The nanoparticles were characterized using transmission electron microscopy, X-ray diffraction, elemental analysis, atomic absorption, energy dispersive X-ray, Fourier transform infrared, and UV–Visible spectrophotometry. The obtained nanoparticles were uniform and spherical with average size of 2–16nm. It was found that increasing the Ag content in the chitosan-Ag based films tends to decrease their equilibrium swelling values. The nanoparticles also demonstrated a relatively high antibacterial activity against Bacillus thuringiensis and Pseudomonas aeruginosa bacteria as compared to that of chitosan and the antibacterial activity increased with increasing the nanoparticle concentration. The obtained results revealed that the prepared nanoparticles could be tailored and used in various biomedical applications.