Biosilica formation in spicules of the sponge Suberites domuncula: Synchronous expression of a gene cluster

• Published in: Genomics (San Diego, Calif.) Vol. 85; no. 6; pp. 666 - 678
• Main Authors: Schröder, Heinz C., Perovic-Ottstadt, Sanja, Grebenjuk, Vladislav A., Engel, Sylvia, Müller, Isabel M., Müller, Werner E.G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2005
• Subjects: Animals; Base Sequence; Cathepsins - biosynthesis; Cathepsins - genetics; Enzymes - biosynthesis; Enzymes - genetics; Gene cluster; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Molecular Sequence Data; Morphogenesis; Porifera; Silicatein; Silicic Acid - pharmacology; Spicules; Sponges; Suberites - genetics; Suberites - physiology; Suberites - ultrastructure; Suberites domuncula; Morphogenesis; Gene cluster; Sponges; Suberites domuncula; Silicatein; Spicules
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2005.02.005

The formation of spicules is a complicated morphogenetic process in sponges (phylum Porifera). The primmorph system was used to demonstrate that in the demosponge Suberites domuncula the synthesis of the siliceous spicules starts intracellularly and is dependent on the concentration of silicic acid. To understand spicule formation, a cluster of genes was isolated. In the center of this cluster is the silicatein gene, which codes for the enzyme that synthesizes spicules. This gene is flanked by an ankyrin repeat gene at one side and by a tumor necrosis factor receptor-associated factor and a protein kinase gene at the other side. All genes are strongly expressed in primmorphs and intact animals after exposure to silicic acid, and this expression is restricted to those areas where the spicule formation starts or where spicules are maintained in the animals. Our observations suggest that in S. domuncula a coordinated expression of physically linked genes is essential for the synthesis of the major skeletal elements.