Long noncoding RNA uc003pxg.1 regulates endothelial cell proliferation and migration via miR‑25‑5p in coronary artery disease

• Published in: International journal of molecular medicine Vol. 48; no. 2; p. 1
• Main Authors: Li, Ping, Li, Yuan, Chen, Lu, Ma, Xuexing, Yan, Xinxin, Yan, Meina, Qian, Buyun, Wang, Feng, Xu, Jingyi, Yin, Juan, Xu, Guidong, Sun, Kangyun
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.08.2021; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Adult; Aged; Aged, 80 and over; Analysis; Anticoagulants; Antisense RNA; Apoptosis; Atherosclerosis; Base Sequence; Biotechnology industry; Blood pressure; Cancer; Cardiovascular disease; Cell cycle; Cell Cycle - genetics; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell growth; Cell Movement - genetics; Cell Proliferation - genetics; Cells, Cultured; Coronary Artery Disease - genetics; Coronary Artery Disease - metabolism; Coronary Artery Disease - pathology; Coronary heart disease; Coronary vessels; Cyclin-dependent kinases; Development and progression; Disease prevention; Endothelium; Ethylenediaminetetraacetic acid; Female; Gene expression; Gene Expression Profiling - methods; Human Umbilical Vein Endothelial Cells - metabolism; Humans; Kinases; Male; Medical research; MicroRNA; MicroRNAs; MicroRNAs - genetics; Middle Aged; Plasma; RNA, Long Noncoding - genetics; RNA-Seq - methods; Scientific equipment and supplies industry; Sequence Homology, Nucleic Acid; United States; China; coronary artery disease; peripheral blood mononuclear cells; long noncoding RNA; cell proliferation and migration
• ISSN: 1107-3756; 1791-244X
• DOI: 10.3892/ijmm.2021.4993

Long noncoding RNAs (lncRNAs) have been reported to be associated with the progression of coronary artery disease (CAD). In our previous study, the levels of lncRNA were upregulated in patients with CAD compared with those in control subjects. However, the role and underlying mechanism of the effects of in CAD remain unknown. Therefore, the aim of the present study was to investigate the expression pattern and biological function of in CAD. First, expression levels were assessed in peripheral blood mononuclear cells isolated from patients with CAD by reverse transcription‑quantitative (RT‑q)PCR. The results demonstrated that the levels of were significantly upregulated (~4.6‑fold) in samples from 80 patients with CAD compared with those in 80 healthy subjects. Subsequently, the present study demonstrated that small interfering RNA‑mediated knockdown inhibited human umbilical vein endothelial cell (HUVEC) proliferation and migration, which was analyzed using the Cell Counting Kit‑8, cell cycle, EdU and Transwell assays. Additionally, the results of RT‑qPCR and western blot analyses revealed that regulated the mRNA and protein levels of cyclin D1 and cyclin‑dependent kinase. Through high‑throughput sequencing and dual‑luciferase reporter assays, the present study demonstrated that microRNA (miR)‑25‑5p was a downstream target of . Further experiments verified that regulated HUVEC proliferation and migration via . The results of the present study may enhance the current understanding of the role of lncRNA in CAD.