Transcriptional activation of Igl, the gene for indole formation in Zea mays: a structure–activity study with elicitor-active N-acyl glutamines from insects
The indole-3-glycerol phosphate lyase Igl is the structural gene of volatile indole biosynthesis in the tritrophic interaction in maize. The gene is activated on transcriptional level with the same kinetics and to the same level by the fatty acid–amino acid conjugates (FAC's) volicitin (17 S)-(...
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Published in | Phytochemistry (Oxford) Vol. 65; no. 8; pp. 1047 - 1055 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier Ltd
01.04.2004
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The indole-3-glycerol phosphate lyase
Igl is the structural gene of volatile indole biosynthesis in the tritrophic interaction in maize. The gene is activated on transcriptional level with the same kinetics and to the same level by the fatty acid–amino acid conjugates (FAC's) volicitin (17
S)-(
N-(17-hydroxylinolenoyl)-
l-glutamine) and
N-linolenoyl-
l-glutamine. Both conjugates are present in the regurgitates of herbivorous caterpillars. Modifications of the fatty acid moiety of the FACs greatly reduces the elicitation of
Igl and only the
l-stereo-isomer of the FACs shows biological activity in the system. Volicitin treatment leads to a fast increase of
AOS and
AOC transcription levels and methyl jasmonate application induces
Igl transcription. Hence, the induction of jasmonate biosynthesis appears to be an integral part of the elicitor mediated increase of
Igl gene transcription.
The structural requirements of fatty acid–amino acid conjugates for induction of
Igl transcription were investigated. A link to jasmonate signaling is described. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0031-9422 1873-3700 |
DOI: | 10.1016/j.phytochem.2003.11.021 |