Endophytic fungi from leaves of Centella asiatica: occurrence and potential interactions within leaves

• Published in: Antonie van Leeuwenhoek Vol. 93; no. 1-2; pp. 27 - 36
• Main Authors: Rakotoniriana, E. F, Munaut, F, Decock, C, Randriamampionona, D, Andriambololoniaina, M, Rakotomalala, T, Rakotonirina, E. J, Rabemanantsoa, C, Cheuk, K, Ratsimamanga, S. U, Mahillon, J, El-Jaziri, M, Quetin-Leclercq, J, Corbisier, A. M
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.02.2008; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Apiaceae; Ascomycetes; Ascomycota - classification; Ascomycota - genetics; Biological and medical sciences; Biomedical and Life Sciences; Centella - microbiology; Centella asiatica; Colletotrichum; Colletotrichum - classification; Colletotrichum - genetics; DNA, Ribosomal Spacer - genetics; Endophytes; Fundamental and applied biological sciences. Psychology; Fungi; Glomerella; Glomerella cingulata; Guignardia; Life Sciences; Medical Microbiology; Metabolites; Microbiology; Molecular Sequence Data; Mycology; Original Paper; Phialophora; Plant diseases; Plant Leaves - microbiology; Plant pathology; Plant Sciences; Sequence Analysis, DNA; Soil Science & Conservation; Systematics; Taxa; Malagasy Republic; Africa; Endophytic fungi; Xylariaceae; Plant pathogen; Taxonomy; Leaf area; Medicinal plant; Fungi; Dicotyledones; Angiospermae; Centella asiatica; Colletotrichum higginsianum; Endophyte; Isolate; Spermatophyta; Umbelliferae
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-007-9176-0

Fungal endophytes were isolated from leaves of Centella asiatica (Apiaceae) collected at Mangoro (middle eastern region of Madagascar, 200 km from Antananarivo). Forty- five different taxa were recovered. The overall foliar colonization rate was 78%. The most common endophytes were the non-sporulating species 1 (isolation frequency IF 19.2%) followed by Colletotrichum sp.1 (IF 13.2%), Guignardia sp. (IF 8.5%), Glomerella sp. (IF 7.7%), an unidentified ascomycete (IF 7.2%), the non-sporulating species 2 (IF 3.7%) and Phialophora sp. (IF 3.5%). Using sequences of the ribosomal DNA internal transcribed spacer (ITS) regions, major endophytes (IF > 7%) were identified as xylariaceous taxa or as Colletotrichum higginsianum, Guignardia mangiferae and Glomerella cingulata. Results from in vitro fungal disk experiments showed a strong inhibitory activity of the xylariaceous non-sporulating species 1 against G. mangiferae and C. higginsianum and of C. higginsianum against G. mangiferae. This can be explained by antagonism between dominant taxa.