Circular RNA circ‑CCT3 promotes hepatocellular carcinoma progression by regulating the miR‑1287‑5p/TEAD1/PTCH1/LOX axis

• Published in: Molecular medicine reports Vol. 23; no. 5
• Main Authors: Lin, Wennan, Zhang, Tianyu, Ding, Guoxu, Hao, Liguo, Zhang, Bingquan, Yu, Jing, Pang, Yu, Geng, Feng, Zhan, Lan, Zhou, Minglu, Yan, Qiyu, Wang, Yuguang, Zheng, Chunlei, Li, Hui
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.05.2021; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Acridine orange; Adult; Aged; Apoptosis; Apoptosis - genetics; Bioinformatics; Carcinogenesis; Carcinogenesis - genetics; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - pathology; Care and treatment; Cell Line, Tumor; Cell Proliferation - genetics; Chemotherapy; Cholecystokinin; Chromatin; Circular RNA; Deoxyribonucleic acid; Development and progression; Disease Progression; DNA; DNA microarrays; DNA-Binding Proteins - genetics; Ethidium bromide; Female; Flow cytometry; Gene expression; Gene Expression Regulation, Neoplastic - genetics; Genetic aspects; Health aspects; Hepatocellular carcinoma; Hepatoma; Humans; Immunoprecipitation; Liver cancer; Liver Neoplasms - genetics; Liver Neoplasms - pathology; Lysyl oxidase; Male; MicroRNA; MicroRNAs - genetics; Middle Aged; Nuclear Proteins - genetics; Patched protein; Patched-1 Receptor - genetics; Prognosis; Protein-Lysine 6-Oxidase - genetics; Proteins; Reverse transcription; RNA, Circular - genetics; Transcription factors; Transcription Factors - genetics; Wound healing; China
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2021.12014

Hepatocellular carcinoma (HCC) is characterized by a poor prognosis because of its insensitivity to radiation and chemotherapy. Recently, circular RNAs (circRNAs) have been found to serve important roles in hepatocellular carcinogenesis. circ‑CCT3, a novel circRNA, was screened from the differential tissue expression results of a circRNA microarray. Relative expression levels of circ‑CCT3 in specimens and cell lines were evaluated by reverse transcription‑quantitative PCR and the relationship between circ‑CCT3 and prognosis was analyzed by Kaplan‑Meier curves. The oncogenic role of circ‑CCT3 was confirmed in HCC cells through a cell counting kit‑8 (CCK‑8) assay, a colony formation assay, acridine orange/ethidium bromide double fluorescence staining, flow cytometry, a wound‑healing assay and a Transwell assay. Bioinformatics prediction and luciferase reporter assays validated that circ‑CCT3 facilitated HCC progression through the miR‑1287‑5p/TEA domain transcription factor 1 (TEAD1) axis. TEAD1 could then directly activate patched 1 and lysyl oxidase transcription, as analyzed by chromatin immunoprecipitation and luciferase reporter assays. The present study identified a novel circRNA, circ‑CCT3, which may be used as a potential therapeutic target for HCC.