Prognostic value of the expression and localization of cell proliferation and apoptosis markers in unicystic ameloblastomas

• Published in: Scientific reports Vol. 14; no. 1; p. 3856
• Main Authors: Vieira Costa, Rebeca, Balbinot, Karolyny Martins, da Silveira, Gabriela Cristina Avertano Rocha, Kataoka, Maria Sueli da Silva, de Menezes, Silvio Augusto Fernandes, Freitas, Vanessa Morais, Vasconcelos, Ana Carolina Uchoa, Etges, Adriana, Martins Montalli, Victor Angelo, Santos, Fabricio Passador, Alves Júnior, Sérgio de Melo, Jaeger, Ruy Gastaldoni, Pinheiro, João de Jesus Viana
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.02.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 692/420; 692/420/755; Ameloblastoma; Apoptosis; Caspase-3; Cell growth; Cell proliferation; Follicles; Humanities and Social Sciences; Immunohistochemistry; Localization; multidisciplinary; Parenchyma; Pathology; Science; Science (multidisciplinary); Immunohistochemistry; Cell proliferation; Ameloblastoma; Pathology; Apoptosis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-54132-7

The aim of this study was to verify whether the expression of cell proliferation and apoptosis markers in different types of unicystic ameloblastoma (UA) is associated with the location of neoplastic cells. Immunohistochemical study with a sample of 32 cases of UA, 11 cases of conventional ameloblastoma (CAM) and ten dental follicles (DF) cases was performed. Cell proliferation was assessed using Ki-67 status, and apoptosis by caspase-3 expression. Mural UA (MUA) showed a higher immunostaining of Ki-67 ( p  < 0.05) and a lower immunostaining of Caspase-3 ( p  < 0.05) compared with luminal and intraluminal subtypes of UA and CAM. The neoplastic cells of the MUA’s cystic capsule showed a higher expression of Ki-67 protein ( p  < 0.0001) and a lower expression of Caspase-3 ( p  < 0.0001) compared with the lumen. DF showed lower Ki-67 and Caspase-3 immunostaining ( p  < 0.05) than neoplasms. The higher immunoexpression of Ki-67 and the lower immunoexpression of Caspase-3 in MUA, in the parenchyma cells within the cystic capsule, suggest an association between the biological behaviour and location of neoplastic cells in a tumour.