Agglutination of bacterial cells of Clostridium innocuum, Bifidobacterium longum, and Micrococcus luteus by lactoferrin and ovotransferrin

The agglutination of bacterial cells in Clostridium innocuum, Bifidobacterium longum, and Micrococcus luteus by lactoferrin (Lf) and ovotransferrin (OTf) was observed by measuring the absorbance of the cells in suspension and also under a phase-contrast microscope. Lf had the ability to agglutinate...

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Published inBioscience, biotechnology, and biochemistry Vol. 58; no. 4; pp. 722 - 726
Main Authors Tomita, S. (Tamagawa Univ., Machida, Tokyo (Japan)), Matsue, M, Matsuyama, J, Kiyosawa, I
Format Journal Article
LanguageEnglish
Published Tokyo Taylor & Francis 1994
Japan Society for Bioscience Biotechnology and Agrochemistry
Oxford University Press
Subjects
Bacteriology
BIFIDOBACTERIUM
Bifidobacterium longum
Biological and medical sciences
CLOSTRIDIUM
Fundamental and applied biological sciences. Psychology
GLOBULINAS
GLOBULINE
LACTOFERRINAS
LACTOFERRINE
Microbiology
MICROCOCCUS
Micrococcus luteus
Miscellaneous
REACCIONES DE AGLUTINACION
REACTION D'AGGLUTINATION
Ferroprotein
Actinomycetaceae
Ovotransferrin
Clostridiales
Bifidobacterium longum
Clostridium innocuum
Micrococcus luteus
Actinomycetales
Suspension culture
Lactoferrin
Structure activity relation
Clostridiaceae
Bacteria
Micrococcales
Actinomycetes
Micrococcaceae
Agglutination
Mechanism of action
Comparative study
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Summary:The agglutination of bacterial cells in Clostridium innocuum, Bifidobacterium longum, and Micrococcus luteus by lactoferrin (Lf) and ovotransferrin (OTf) was observed by measuring the absorbance of the cells in suspension and also under a phase-contrast microscope. Lf had the ability to agglutinate C. innocuum and M. luteus, but not B. longum. However, OTf and other proteins from bovine and human milk and from hen's egg white did not affect the agglutination of these bacteria. Lf, when the lysine or arginine residues were chemically modified, completely lost the ability to agglutinate the bacterial cells, while desialylated Lf enhanced the agglutination of the bacterial cells. The absorbance of the cells in suspension by deglycocylated Lf was the same as that by untreated Lf after 1 min, but was markedly decreased after 30 min
Bibliography:F60
9500985
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.58.722