Nonpathogenic Pseudomonas syringae derivatives and its metabolites trigger the plant “cry for help” response to assemble disease suppressing and growth promoting rhizomicrobiome

• Published in: Nature communications Vol. 15; no. 1; pp. 1907 - 14
• Main Authors: Liu, Yunpeng, Zhang, Huihui, Wang, Jing, Gao, Wenting, Sun, Xiting, Xiong, Qin, Shu, Xia, Miao, Youzhi, Shen, Qirong, Xun, Weibing, Zhang, Ruifu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 38; 38/77; 42; 45; 49; 49/23; 49/88; 631/158/2456; 631/326/41/2533; 631/449/2169/2674; 64; Animals; Arabidopsis; Bacteria; Estrus; Exudation; Hot Temperature; Humanities and Social Sciences; Immune response; Immune system; Metabolites; multidisciplinary; Pathogens; Plant bacterial diseases; Pseudomonas; Pseudomonas syringae; Rhizosphere; Science; Science (multidisciplinary); Soil; Soils; Tomatoes
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-46254-3

Plants are capable of assembling beneficial rhizomicrobiomes through a “cry for help” mechanism upon pathogen infestation; however, it remains unknown whether we can use nonpathogenic strains to induce plants to assemble a rhizomicrobiome against pathogen invasion. Here, we used a series of derivatives of Pseudomonas syringae pv. tomato DC3000 to elicit different levels of the immune response to Arabidopsis and revealed that two nonpathogenic DC3000 derivatives induced the beneficial soil-borne legacy, demonstrating a similar “cry for help” triggering effect as the wild-type DC3000. In addition, an increase in the abundance of Devosia in the rhizosphere induced by the decreased root exudation of myristic acid was confirmed to be responsible for growth promotion and disease suppression of the soil-borne legacy. Furthermore, the “cry for help” response could be induced by heat-killed DC3000 and flg22 and blocked by an effector triggered immunity (ETI) -eliciting derivative of DC3000. In conclusion, we demonstrate the potential of nonpathogenic bacteria and bacterial elicitors to promote the generation of disease-suppressive soils. Upon pathogen attack, plants can trigger the “cry for help” response and assemble beneficial rhizobacteria. Here, the authors use nonpathogenic Pseudomonas syringae DC3000 derivatives to elicit a similar “cry for help” response as the wild-type pathogenic DC3000 in Arabidopsis.