Nano- and microparticles as adjuvants in vaccine design: Success and failure is related to host natural antibodies

• Published in: Vaccine Vol. 24; no. 42; pp. 6534 - 6541
• Main Authors: Sinyakov, Michael S., Dror, Moti, Lublin-Tennenbaum, Tammy, Salzberg, Samuel, Margel, Shlomo, Avtalion, Ramy R.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 30.10.2006; Elsevier; Elsevier Limited
• Subjects: Acquired antibodies; Acrolein - chemistry; Acrolein - immunology; Adjuvants, Immunologic - chemistry; Adjuvants, Immunologic - pharmacology; Aeromonas salmonicida; Aeromonas salmonicida - growth & development; Aeromonas salmonicida - immunology; Aluminum Compounds - pharmacology; Animal species; Animals; Antibodies - chemistry; Antibodies - immunology; Antigens; Antigens - immunology; Antigens, Bacterial - chemistry; Antigens, Bacterial - immunology; Applied microbiology; Aqueous solutions; Bacteriology; Biological and medical sciences; Carassius auratus; Cattle; Chemistry, Pharmaceutical; Drug Design; Emulsions; Fundamental and applied biological sciences. Psychology; Goldfish - immunology; Immune response; Immune system; Indicators and Reagents; Mammals; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Molecular weight; Nanoparticles; Nanoparticles - chemistry; Natural antibodies; Phosphatase; Polymers - chemistry; Sepharose - chemistry; Serum Albumin, Bovine - immunology; Surfactants; Vaccines; Vaccines - administration & dosage; Vaccines - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Natural antibodies; Aeromonas salmonicida; Acquired antibodies; Antibody; Immunological adjuvant; Bacteria; Vibrionaceae; Vaccine; Failure
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2006.06.021

Bovine serum albumin (BSA) and the surface A-layer protein (AP) of an atypical strain of fish bacterial pathogen Aeromonas salmonicida were covalently linked with polymeric nano- and microparticles, and antigenicity of the resulted conjugates was compared in mice and goldfish. Distinct albeit different levels of natural BSA and AP antibodies were present in both animal species. Significant stimulation of the anti-AP antibody response in mice strikingly contrasted to unresponsiveness or even suppression in fish. The results negatively correlate with the levels of respective natural antibodies in the host and are discussed in context of problems related to fish vaccination. The work reinforces the instructive role of natural antibodies in adaptive immune response.