Metabolic Discrimination between Adventitious Roots and Standard Medicinal Part of Atractylodes macrocephala Koidz. Using FT-IR Spectroscopy

• Published in: Plants (Basel) Vol. 12; no. 9; p. 1821
• Main Authors: Choi, So Yeon, Ku, Seong Sub, Ahn, Myung Suk, So, Eun Jin, Kim, HyeRan, Park, Sang Un, Lee, Moon-Soon, Kang, Young Min, Min, Sung Ran, Kim, Suk Weon
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.04.2023; MDPI
• Subjects: Atractylodes macrocephala; Butyric acid; Cell culture; Discriminant analysis; Discrimination; Fourier transforms; Herbal medicine; Indole-3-butyric acid; indole-3-butyric acid (IBA); Infrared analysis; Infrared spectroscopy; Inorganic salts; Investigations; liquid culture; medicinal plants; Metabolism; Metabolites; partial least square discriminant analysis (PLS-DA); principal component analysis (PCA); Principal components analysis; Roots; indole-3-butyric acid (IBA); medicinal plants; partial least square discriminant analysis (PLS-DA); liquid culture; principal component analysis (PCA)
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12091821

This study aims to examine the metabolic discrimination between in vitro grown adventitious roots and the standard medicinal parts of . To achieve this goal, firstly, in vitro culture conditions of adventitious roots such as indole-3-butyric acid (IBA) concentrations, types of media, inorganic salt strength of culture medium, and elicitor types and concentrations were optimized. The optimal culture conditions for proliferation of adventitious roots was found to consist of Murashige and Skoog (MS) medium containing 5 mg L IBA. Whole cell extracts from adventitious roots and the standard medicinal parts of were subjected to Fourier transform infrared spectroscopy (FT-IR). Principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) from FT-IR spectral data showed that adventitious roots and standard medicinal parts were clearly distinguished in the PCA and PLS-DA score plot. Furthermore, the overall metabolite pattern from adventitious roots was changed depending on the dose-dependent manner of chemicals. These results suggest that FT-IR spectroscopy can be applied as an alternative tool for the screening of higher metabolic root lines and for discriminating metabolic similarity between in vitro grown adventitious roots and the standard medicinal parts. In addition, the adventitious roots proliferation system established in this study can be directly applied as an alternative means for the commercial production of