Paraprobiotics and Postbiotics of Lactobacillus delbrueckii CIDCA 133 Mitigate 5-FU-Induced Intestinal Inflammation

• Published in: Microorganisms (Basel) Vol. 10; no. 7; p. 1418
• Main Authors: Batista, Viviane Lima, De Jesus, Luís Cláudio Lima, Tavares, Laísa Macedo, Barroso, Fernanda Lima Alvarenga, Fernandes, Lucas Jorge da Silva, Freitas, Andria dos Santos, Americo, Monique Ferrary, Drumond, Mariana Martins, Mancha-Agresti, Pamela, Ferreira, Enio, Laguna, Juliana Guimarães, Alcantara, Luiz Carlos Júnior, Azevedo, Vasco
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 14.07.2022; MDPI
• Subjects: 5-Fluorouracil; Anti-inflammatory agents; cell-free supernatant; Chemotherapy; Chronic fatigue syndrome; Cytokines; Damage; Epithelium; Health risks; Heat; heat-killed bacteria; IL-1β; immunomodulation; Immunoregulation; Inflammation; Interleukin 10; Interleukin 12; intestinal damage; Intestine; Laboratory animals; Lactobacilli; Lactobacillus delbrueckii; Leukocytes (neutrophilic); Markers; Microbiota; Microorganisms; Mucositis; Probiotics; Proteins; R&D; Research & development; Side effects; Small intestine; TLR2 protein; Toll-like receptors; Tumor necrosis factor; Brazil; Rio de Janeiro Brazil; St Louis Missouri; United States > US
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10071418

Intestinal mucositis is a commonly reported side effect in oncology practice. Probiotics are considered an excellent alternative therapeutic approach to this debilitating condition; however, there are safety questions regarding the viable consumption of probiotics in clinical practice due to the risks of systemic infections, especially in immune-compromised patients. The use of heat-killed or cell-free supernatants derived from probiotic strains has been evaluated to minimize these adverse effects. Thus, this work evaluated the anti-inflammatory properties of paraprobiotics (heat-killed) and postbiotics (cell-free supernatant) of the probiotic Lactobacillus delbrueckii CIDCA 133 strain in a mouse model of 5-Fluorouracil drug-induced mucositis. Administration of paraprobiotics and postbiotics reduced the neutrophil cells infiltrating into the small intestinal mucosa and ameliorated the intestinal epithelium architecture damaged by 5-FU. These ameliorative effects were associated with a downregulation of inflammatory markers (Tlr2, Nfkb1, Il12, Il17a, Il1b, Tnf), and upregulation of immunoregulatory Il10 cytokine and the epithelial barrier markers Ocln, Cldn1, 2, 5, Hp and Muc2. Thus, heat-killed L. delbrueckii CIDCA 133 and supernatants derived from this strain were shown to be effective in reducing 5-FU-induced inflammatory damage, demonstrating them to be an alternative approach to the problems arising from the use of live beneficial microorganisms in clinical practice.