Effect of Dietary Restriction on the Age-Dependent Changes in the Expression of Antioxidant Enzymes in Rat Liver

• Published in: The Journal of nutrition Vol. 120; no. 6; pp. 602 - 609
• Main Authors: Rao, Govinda, Xia, Erning, Nadakavukaren, Mathew J., Richardson, Arlan
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.06.1990; American Society for Nutritional Sciences
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; AGING; Aging - metabolism; Animals; Biological and medical sciences; CATALASA; CATALASE; Catalase - genetics; Catalase - metabolism; Diet; Energy Intake; ENVEJECIMIENTO; ENZYMIC ACTIVITY; Feeding. Feeding behavior; FOIE; Free Radicals; Fundamental and applied biological sciences. Psychology; GENE; Gene Expression; GENES; GLUTATHIONE PEROXIDASE; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; HIGADO; INANICION; INANITION; Lipid Peroxidation; Lipofuscin - metabolism; LIVER; Liver - enzymology; Liver - pathology; Liver - ultrastructure; Male; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; RAT; RATA; RATS; Rats, Inbred F344; RNA, Messenger - metabolism; STARVATION; SUPEROXIDE DISMUTASE; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; SUPEROXIDO DISMUTASA; SUPEROXYDE DISMUTASE; Vertebrates: anatomy and physiology, studies on body, several organs or systems; VIEILLISSEMENT; aging; rats; superoxide dismutase; catalase; glutathione peroxidase; Glutathione peroxidase; Rat; Digestive system; Enzyme; Liver; Rodentia; Superoxide dismutase; Gene expression; Free radical; Feeding; Vertebrata; Mammalia; Catalase; Restricted diet; Age
• ISSN: 0022-3166; 1541-6100
• DOI: 10.1093/jn/120.6.602

The effects of aging and dietary restriction on the expression of several enzymes (superoxide dismutase, catalase and glutathione peroxidase) that are involved in free radical detoxification were studied in liver tissue from male Fischer F344 rats. The expression (i.e., activities and mRNA levels) of superoxide dismutase (Cu-Zn) and catalase decreased with age in liver. Dietary restriction (40% restriction of energy intake) increased the activities of superoxide dismutase (24 to 38%) and catalase (64 to 75%) in liver at 21 and 28 mo of age. Glutathione peroxidase activity in liver of diet-restricted rats was significantly higher (37%) at 28 mo of age than that of rats fed ad libitum. The age-related changes in the relative levels of mRNA for superoxide dismutase, catalase and glutathione peroxidase paralleled the changes in the activities of these enzymes in rats fed ad libitum or rats fed the restricted diet. Thus, the changes in the activities of superoxide dismutase, catalase and glutathione peroxidase with age and dietary restriction appear to arise from changes in the levels of mRNAs coding for these enzymes. Free radical damage, as measured by thiobarbituric acid-reactive material and lipofuscin accumulation, was lower in diet-restricted rats than in rats fed ad libitum.