Enhanced textile dye decolorization by marine-derived basidiomycete Peniophora sp. CBMAI 1063 using integrated statistical design

• Published in: Environmental science and pollution research international Vol. 23; no. 9; pp. 8659 - 8668
• Main Authors: Bonugli-Santos, Rafaella C., Vieira, Gabriela A. L., Collins, Catherine, Fernandes, Thaís Cristina C., Marin-Morales, Maria Aparecida, Murray, Patrick, Sette, Lara D.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2016; Springer Nature B.V
• Subjects: Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Basidiomycetes; Basidiomycota - metabolism; Basidiomycota - physiology; Biodegradation; Biodegradation, Environmental; Biotechnology; Coloring Agents - metabolism; Decolorization; Design; Dyes; Earth and Environmental Science; Ecotoxicology; Effluents; Environment; Environmental Chemistry; Environmental Health; Environmental science; Enzymatic activity; Enzymes; Fungi; Gene expression; Laccase - genetics; Laccase - metabolism; Lignin peroxidase; Manganese; Manganese peroxidase; Metabolites; Microorganisms; MnP gene; Optimization; Organic carbon; Peroxidases - genetics; Peroxidases - metabolism; Research Article; Salinity; Statistics; Studies; Textile industry; Textiles; Total organic carbon; Waste Disposal, Fluid - methods; Waste Water - chemistry; Waste Water Technology; Water Management; Water Pollutants, Chemical - metabolism; Water Pollution Control; Brazil; Textile dye degradation; Marine-derived fungi; Experimental design; Microbial biotechnology; Ligninolytic enzymes; Dye decolorization
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-016-6053-2

In the present study, the biotechnological potential of the marine-derived fungus Peniophora sp. CBMAI 1063 was investigated in relation to Reactive Black 5 (RB5) dye decolorization and degradation using an integrated statistical design composed of Plackett-Burman design (P&B), central composite design (CCD), and response surface methodology (RSM). RB5 dye was effectively decolorized (94 %) in saline conditions, without any detection of mutagenic compounds, and simultaneously, 57 % of total organic carbon (TOC) was removed in 7 days. The activity of lignin peroxidase (LiP) was not detected during the process. The gene expression of laccase (Lac) and manganese peroxidase (MnP) enzymes produced during the process was evaluated, and results from this experiment coupled with LC-MS analyses revealed that in the early stage of dye decolorization, a higher MnP gene expression and significant enzymatic activity was detected in Peniophora sp. CBMAI 1063 with the formation of p-Base and TAHNDS compounds. This paper reports innovative data related to the textile dye decolorization by the marine-derived basidiomycete Peniophora sp. CBMAI 1063, showing the metabolites formed and enzymatic action throughout the process in saline condition. The strategy used showed to be an efficient statistical approach that provides an attractive solution for the screening and simultaneous optimization of the degradation process.