Organic Anion Transporting Polypeptide 3A1 (OATP3A1)-Gated Bio-Orthogonal Labeling of Intracellular Proteins

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 6; p. 2521
• Main Authors: Németh, Krisztina, László, Zsófia, Biró, Adrienn, Szatmári, Ágnes, Cserép, Gergely B, Várady, György, Bakos, Éva, Özvegy-Laczka, Csilla, Kele, Péter
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.03.2023; MDPI
• Subjects: Anions; Cell membranes; cell permeability; Confocal microscopy; Drug interactions; Fluorescence microscopy; Fluorescent Dyes; Fluorescent indicators; Intracellular; intracellular labeling; Investigations; large Stokes shift fluorescent probes; live cell bio-orthogonal modification; Microscopy; organic anion transporter polypeptide 3A1; Organic Anion Transporters - metabolism; Organic anion transporting polypeptide; Pharmaceutical industry; Polypeptides; Probes; Protein transport; Proteins; Proteins - metabolism; self-labeling enzyme tags; Hungary; cell permeability; self-labeling enzyme tags; large Stokes shift fluorescent probes; confocal microscopy; intracellular labeling; flow cytometry; live cell bio-orthogonal modification; organic anion transporter polypeptide 3A1; super-resolution microscopy
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28062521

Organic anion transporting polypeptides (OATPs) were found to readily deliver membrane impermeable, tetrazine bearing fluorescent probes into cells. This feature was explored in OATP3A1 conditioned bio-orthogonal labeling schemes of various intracellular proteins in live cells. Confocal microscopy and super-resolution microscopy (STED) studies have shown that highly specific and efficient staining of the selected intracellular proteins can be achieved with the otherwise non-permeable probes when OATP3A1 is present in the cell membrane of cells. Such a transport protein linked bio-orthogonal labeling scheme is believed to be useful in OATP3A1 activity-controlled protein expression studies in the future.