Quantitative diffusion MRI of hippocampus as a surrogate marker for post-traumatic epileptogenesis

• Published in: Brain (London, England : 1878) Vol. 130; no. 12; pp. 3155 - 3168
• Main Authors: Kharatishvili, Irina, Immonen, Riikka, Gröhn, Olli, Pitkänen, Asla
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.12.2007; Oxford Publishing Limited (England)
• Subjects: Animals; Biological and medical sciences; Brain Injuries - pathology; Cerebral Cortex - pathology; Convulsants; Diffusion Magnetic Resonance Imaging - methods; Disease Models, Animal; Disease Progression; Disease Susceptibility; Electrodes, Implanted; Electroencephalography; Epilepsy, Post-Traumatic - diagnosis; Hippocampus - pathology; Injuries of the nervous system and the skull. Diseases due to physical agents; Investigative techniques, diagnostic techniques (general aspects); magnetic resonance imaging; Male; Medical sciences; Nervous system; Neurology; Pentylenetetrazole; post-traumatic epilepsy; Prognosis; Radiodiagnosis. Nmr imagery. Nmr spectrometry; Rats; Rats, Sprague-Dawley; surrogate marker; Survival Analysis; Trauma Severity Indices; Traumas. Diseases due to physical agents; traumatic brain injury; video-EEG monitoring; traumatic brain injury; post-traumatic epilepsy; magnetic resonance imaging; surrogate marker; video-EEG monitoring; Nervous system diseases; Epilepsy; Central nervous system; Electrophysiology; Electroencephalography; Nuclear magnetic resonance imaging; Encephalon; Cerebral disorder; Central nervous system disease; Head trauma; Diffusion; Hippocampus; Monitoring
• ISSN: 0006-8950; 1460-2156
• DOI: 10.1093/brain/awm268

The need to use animal models to develop imaging markers that could be linked to electrophysiological abnormalities in epilepsy and able to predict epileptogenicity in human studies is widely acknowledged. This study aimed to investigate the value of early magnetic resonance imaging (MRI) in predicting the long-term increased seizure susceptibility in the clinically relevant model of post-traumatic epilepsy (PTE). Moderate traumatic brain injury (TBI) was induced by lateral fluid-percussion in two groups of adult rats (34 injured, 16 controls). In Experiment 1, MRI follow-up was performed using a 4.7 T magnet at 3 h, 3 days, 9 days, 23 days, 2 months, 3 months and 6 months after TBI. T2 and 1/3 of the trace of the diffusion tensor (Dav) were quantified from a single slice using a fast spin-echo sequence. In Experiment 2, MRI was performed at 7 and 11 months post-injury. In both groups, seizure susceptibility was tested by injecting a single dose of pentylenetetrazol at 12 months post-injury. Electrographic and behavioural responses were monitored for 1 h. Total number of spikes, total number of epileptiform discharges (EDs) and latency to first spike were measured. Finally, the severity of mossy fibre sprouting was evaluated. In both experiments, EEG parameters such as total number of spikes or EDs proved to be reliable indicators of increased seizure susceptibility in injured animals when compared to controls (P < 0.05). In the hippocampus ipsilateral to TBI, Dav correlated with these EEG parameters at both early (3 h), and chronic (23 days, 2, 3, 6, 7 and 11 months) time points after TBI, as well as with the density of mossy fibre sprouting. These results for the first time demonstrate that quantitative diffusion MRI can serve as a tool to facilitate prediction of increased seizure susceptibility in a clinically relevant model of human PTE.