Overcoming Difficulties in Molecular Biological Analysis through a Combination of Genetic Engineering, Genome Editing, and Genome Analysis in Hexaploid Chrysanthemum morifolium

Chrysanthemum is one of the most commercially important ornamental plants globally, of which many new varieties are produced annually. Among these new varieties, many are the result of crossbreeding, while some are the result of mutation breeding. Recent advances in gene and genome sequencing techno...

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Bibliographic Details
Published inPlants (Basel) Vol. 12; no. 13; p. 2566
Main Authors Sasaki, Katsutomo, Tanaka, Tsuyoshi
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 06.07.2023
MDPI
Subjects
Biological analysis
Biotechnology
breeding
chrysanthemum
Cross-breeding
Editing
Flowers & plants
Gene sequencing
Genetic analysis
Genetic engineering
genome analysis
Genome editing
Genomes
Incompatibility
Mutation
Ornamental plants
Plant breeding
Review
Seeds
Self-incompatibility
Transcriptomes
genome editing
biotechnology
chrysanthemum
genome analysis
hexaploid
transcriptome analysis
genetic engineering
breeding
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Summary:Chrysanthemum is one of the most commercially important ornamental plants globally, of which many new varieties are produced annually. Among these new varieties, many are the result of crossbreeding, while some are the result of mutation breeding. Recent advances in gene and genome sequencing technology have raised expectations about the use of biotechnology and genome breeding to efficiently breed new varieties. However, some features of chrysanthemum complicate molecular biological analysis. For example, chrysanthemum is a hexaploid hyperploid plant with a large genome, while its genome is heterogeneous because of the difficulty of obtaining pure lines due to self-incompatibility. Despite these difficulties, an increased number of reports on transcriptome analysis in chrysanthemum have been published as a result of recent technological advances in gene sequencing, which should deepen our understanding of the properties of these plants. In this review, we discuss recent studies using gene engineering, genome editing, and genome analysis, including transcriptome analysis, to analyze chrysanthemum, as well as the current status of and future prospects for chrysanthemum.
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ISSN:2223-7747
2223-7747
DOI:10.3390/plants12132566