Electronegative Low-Density Lipoprotein is Associated with Dense Low-Density Lipoprotein in Subjects with Different Levels of Cardiovascular Risk

• Published in: Lipids Vol. 45; no. 7; pp. 619 - 625
• Main Authors: de Queiroz Mello, Ana Paula, da Silva, Isis Tande, Oliveira, Aline Silva, Nunes, Valéria Sutti, Abdalla, Dulcineia Saes Parra, Gidlund, Magnus, Damasceno, Nágila Raquel Teixeira
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.07.2010; Springer-Verlag; Springer‐Verlag; Springer Nature B.V
• Subjects: Adult; Aged; Atherosclerosis; Biomedical and Life Sciences; Cardiovascular Diseases - epidemiology; Cardiovascular Diseases - metabolism; Electronegative low‐density lipoprotein; Female; Health risks; Humans; LDL phenotype B; Life Sciences; Lipidology; Lipoproteins, LDL - blood; Lipoproteins, LDL - metabolism; Male; Medical Biochemistry; Medicinal Chemistry; Microbial Genetics and Genomics; Middle Aged; Neurochemistry; Nutrition; Original Article; Oxidation; Risk Factors; Oxidation; Electronegative low-density lipoprotein; LDL phenotype B; Atherosclerosis
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-010-3439-7

Dyslipidemias and physicochemical changes in low-density lipoprotein (LDL) are very important factors for the development of coronary artery disease (CAD). However, pathophysiological properties of electronegative low-density lipoprotein [LDL(−)] remain a controversial issue. Our objective was to investigate LDL(−) content in LDL and its subfractions (phenotypes A and B) of subjects with different cardiovascular risk. Seventy-three subjects were randomized into three groups: normolipidemic (N; n = 30) and hypercholesterolemic (HC; n = 33) subjects and patients with CAD (n = 10). After fasting, blood samples were collected and total, dense and light LDL were isolated. LDL(−) content in total LDL and its subfractions was determined by ELISA. LDL(−) content in total LDL was lower in the N group as compared to the HC (P < 0.001) and CAD (P = 0.006) groups. In the total sample and in those of the N, HC, and CAD groups, LDL(−) content in dense LDL was higher than in light LDL (P = 0.001, 0.001, 0.001, and 0.033, respectively) The impact of LDL(−) on cardiovascular risk was reinforced when LDL(−) content in LDL showed itself to have a positive association with total cholesterol (β = 0.003; P < 0.001), LDL-C (β = 0.003; p < 0.001), and non-HDL-C (β = 0.003; P < 0.001) and a negative association with HDL-C (β = −0.32; P = 0.04). Therefore, LDL(−) is an important biomarker that showed association with the lipid profile and the level of cardiovascular risk.