Analysis of the Human CD10/Neutral Endopeptidase 24.11 Promoter Region: Two Separate Regulatory Elements

• Published in: Blood Vol. 85; no. 11; pp. 3199 - 3207
• Main Authors: Ishimaru, Fumihiko, Shipp, Margaret A.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01.06.1995; The Americain Society of Hematology
• Subjects: Base Sequence; Biological and medical sciences; DNA, Complementary - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic; Genes; Humans; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Neprilysin - genetics; Nucleic Acid Conformation; Organ Specificity; Promoter Regions, Genetic; Regulatory Sequences, Nucleic Acid; RNA Splicing; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Human; 5' terminal-Sequence; Enzyme; Transcription promoter; Stem cell; Hematopoietic cell; Metalloendopeptidases; Characterization; Gene; Hydrolases; Epithelial cell; Regulatory sequence; Neprilysin; Proteinases
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V85.11.3199.bloodjournal85113199

The cell surface zinc metalloproteinase CD10/neutral endopeptidase 24.11 (NEP) is expressed on normal and malignant lymphoid progenitors, granulocytes, and a variety of epithelial cells. To further define the tissue-specific and develop-mentally related expression of CD10/NEP, we have characterized two separate regulatory regions that control the transcription of 5‘ alternatively spliced CD10/NEP transcripts. These type 1 and 2 CD10/NEP regulatory regions are both characterized by the presence of multiple transcription initiation sites and the absence of classic TATA boxes and consensus initiator elements. The purine-rich type 1 regulatory region, which includes 5‘ UTR exon 1 sequence, is characterized by multiple putative PU.1 binding sites and consensus efs-binding motifs. In marked contrast, the GC-rich type 2 regulatory region contains multiple putative Sp1 binding sites, a potential consensus retinoblastoma control element (RCE), and an inverted CCAAT box. In the majority of tissues examined to date, type 2 CD10/NEP transcripts were more abundant; the abundance of type 1 transcripts was more variable, with the highest type 1 levels in fetal thymus and certain lymphoblastic leukemia cell lines.