Performance of the DNA-Citoliq liquid-based cytology system compared with conventional smears

• Published in: Cytopathology (Oxford) Vol. 17; no. 2; pp. 86 - 93
• Main Authors: Alves, V. A. F., Castelo, A., Filho, A. L., Vianna, M. R., Taromaru, E., Namiyama, G., Lorincz, A., Dores, G. B. D.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.04.2006
• Subjects: Carcinoma, Squamous Cell - diagnosis; Carcinoma, Squamous Cell - pathology; Cytodiagnosis - methods; DNA-Citoliq system; Efficiency; Female; HPV; Humans; liquid-based cytology; Predictive Value of Tests; Sensitivity and Specificity; severe dyskaryosis; split-sample study; thin layer cytology; Uterine Cervical Diseases - diagnosis; Vaginal Smears - methods
• ISSN: 0956-5507; 1365-2303
• DOI: 10.1111/j.1365-2303.2006.00279.x

Objective: To evaluate the performance of a new, manual, simplified liquid‐based system, DNA‐Citoliq (Digene Brasil), employed under routine conditions as compared to conventional smears collected from six collaborating private laboratories. Methods: A panel of cytopathologists, who served as the gold standard diagnosis, adjudicated discordant opinions. Results:  Of 3206 pairs of slides considered valid for comparison, there were 3008 in full agreement (93.8%), 112 (3.5%) with one diagnostic category discrepancies, and 86 (2.7%) discordant cases. Among the 288 borderline+ by either method, DNA‐Citoliq detected abnormalities in 243 (84.4%), and conventional smears (CS) detected abnormalities in 178 (61.8%) (McNemar test, P < 0.000), a 36.5% increased detection of borderline+ cases. Conclusions:  For mild dyskaryosis, DNA‐Citoliq detected 176 cases and CS 125 cases (McNemar test, P < 0.000); and for moderate+severe dyskaryosis 66 versus 32 cases respectively (McNemar test, P < 0.000).