A Proteolipid Protein‐Specific Pre‐mRNA (Ppm‐1) Contains Intron 3 and Is Up‐Regulated During Myelination in the CNS

• Published in: Journal of neurochemistry Vol. 74; no. 3; pp. 940 - 948
• Main Authors: Vouyiouklis, Demetrius A., Barrie, Jennifer A., Griffiths, Ian R., Thomson, Christine E.
• Format: Journal Article
• Language: English
• Published: Oxford UK Blackwell Science Ltd 01.03.2000; Blackwell
• Subjects: Animals; Apoproteins - genetics; Base Sequence - genetics; Biological and medical sciences; Brain - physiology; Cell Nucleus - metabolism; DM20; DNA, Recombinant; Dogs; Fish Proteins; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental; Introns - physiology; Mice; Mice, Inbred C3H; Mice, Jimpy; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Myelin Proteolipid Protein - genetics; Myelin Sheath - physiology; Nerve Tissue Proteins; Oligodendroglia - metabolism; Oncorhynchus mykiss; Ppm‐1; Pre‐mRNA; Proteolipid protein; Rats; Rats, Wistar; RNA, Messenger - metabolism; Splicing; Transcription. Transcription factor. Splicing. Rna processing; Up-Regulation; Xenopus laevis; Salmonidae; Fissipedia; Alternative splicing; Carnivora; Rat; Xenopus laevis; Rodentia; Central nervous system; Amphibia; Salientia; Oncorhynchus mykiss; Vertebrata; Myelination; Mammalia; Messenger RNA; Mouse; Pisces; Dog; Proteolipid
• ISSN: 0022-3042; 1471-4159
• DOI: 10.1046/j.1471-4159.2000.0740940.x

: Alternative splicing of the precursor for messenger RNA (pre‐mRNA) is a common process utilised by higher eukaryotes to modulate gene expression. A single primary transcript may generate several proteins with distinct functions, expressed in tissue‐specific, developmental patterns. This article describes an oligodendrocyte‐specific pre‐mRNA product of proteolipid protein gene (Plp) transcription, which is the precursor for Plp but not Dm20 mRNA in the CNS. This Plp‐specific pre‐mRNA (Ppm‐1) includes the intact intron 3 of the Plp gene. It is first expressed during active myelination, and it localises to the nucleus of oligodendrocytes, in both normal and jimpy (jp) murine CNS. In addition to mouse, Ppm‐1 is found also in rat and dog, but not toad or trout. Our work suggests that alternative splicing of the Plp gene primary transcript follows a branching pattern, resulting in the presence of at least one Plp isoform‐specific pre‐mRNA molecule, Ppm‐1. Therefore, Dm20 mRNA may be the product of a divergent set of pre‐mRNA splicing events.