Regulation of the Later Stages of Nodulation Stimulated by IPD3/CYCLOPS Transcription Factor and Cytokinin in Pea Pisum sativum L

• Published in: Plants (Basel) Vol. 11; no. 1; p. 56
• Main Authors: Rudaya, Elizaveta S, Kozyulina, Polina Yu, Pavlova, Olga A, Dolgikh, Alexandra V, Ivanova, Alexandra N, Dolgikh, Elena A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.12.2021; MDPI
• Subjects: Bacteria; Biosynthesis; Comparative analysis; cytokinin; Cytokinins; Developmental stages; Differentiation; Enzymes; Gene expression; Gene regulation; Infections; IPD3/CYCLOPS transcription factor; Kinases; Legumes; Localization; Microscopy; Morphology; Mutants; Nodulation; Nodules; Peas; Pisum sativum; Plant tissues; Primordia; Proteins; Ribonucleic acid; RNA; Signal transduction; Transcription factors; transcriptomic data; IPD3/CYCLOPS transcription factor; cytokinin; Pisum sativum; gene expression; transcriptomic data
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants11010056

The IPD3/CYCLOPS transcription factor was shown to be involved in the regulation of nodule primordia development and subsequent stages of nodule differentiation. In contrast to early stages, the stages related to nodule differentiation remain less studied. Recently, we have shown that the accumulation of cytokinin at later stages may significantly impact nodule development. This conclusion was based on a comparative analysis of cytokinin localization between pea wild type and mutants. However, the role of cytokinin at these later stages of nodulation is still far from understood. To determine a set of genes involved in the regulation of later stages of nodule development connected with infection progress, intracellular accommodation, as well as plant tissue and bacteroid differentiation, the RNA-seq analysis of pea mutant SGEFix -2 ( ) nodules impaired in these processes compared to wild type SGE nodules was performed. To verify cytokinin's influence on late nodule development stages, the comparative RNA-seq analysis of SGEFix -2 ( ) mutant plants treated with cytokinin was also conducted. Findings suggest a significant role of cytokinin in the regulation of later stages of nodule development.