Further characterization of the juxtaglomerular neurons in the mouse main olfactory bulb by transcription factors, Sp8 and Tbx21

• Published in: Neuroscience research Vol. 73; no. 1; pp. 24 - 31
• Main Authors: Kosaka, Toshio, Kosaka, Katsuko
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.05.2012
• Subjects: Animals; Calbindin; Calbindins; Calcium-binding protein; Calretinin; Dendrites; DNA-Binding Proteins - biosynthesis; DNA-Binding Proteins - chemistry; Immunoreactivity; Interneurons; Juxtaglomerular Apparatus - chemistry; Juxtaglomerular Apparatus - enzymology; Juxtaglomerular Apparatus - metabolism; Local circuit neuron; Male; Mice; Mice, Inbred C57BL; Nervous system; Neuroactive substances; Neurons - enzymology; Neurons - metabolism; Nitric Oxide Synthase - biosynthesis; Nitric Oxide Synthase - chemistry; Nitric-oxide synthase; Olfactory bulb; Olfactory Bulb - chemistry; Olfactory Bulb - metabolism; Olfactory system; Projection neuron; S100 Calcium Binding Protein G - biosynthesis; T-Box Domain Proteins - biosynthesis; T-Box Domain Proteins - chemistry; Transcription factors; Transcription Factors - biosynthesis; Transcription Factors - chemistry; Tyrosine 3-monooxygenase; EPL; MOB; Transcription factors; IPL; GL; DAPI; Neuroactive substances; ONL; Olfactory system; CR; GCL; SE; NOS; Projection neuron; TH; Local circuit neuron; CLSM; CB; ML
• ISSN: 0168-0102; 1872-8111
• DOI: 10.1016/j.neures.2012.02.013

► Immunoreactivities of transcription factors Sp8 and Tbx21 were examined in the mouse MOB. ► The intensities of the Sp8 and Tbx21 immunoreactivities were so diverse. ► Sp8 and Tbx21 characterized subpopulations of juxtaglomerular neurons differently. ► Sp8 and Tbx21 further confirmed the heterogeneity of NOS positive cells. Juxtaglomerular neurons in the mouse main olfactory bulb consist of various types of neurons, especially classified by their chemical properties such as transmitter-related molecules and calcium binding proteins. In addition several transcription factors have been revealed to characterize neuronal subpopulations. In this study we examined the immunoreactivities of two transcription factors, Sp8 and Tbx21, in the juxtaglomerular neuronal subpopulations containing calretinin, calbindin, secretagogin, tyrosine hydroxylase (TH) and nitric oxide synthase (NOS). Both Sp8 and Tbx21 immunoreactivities were so diverse in their staining intensities. Almost all calretinin and secretagogin positive neurons were relatively strongly Sp8 positive, whereas none of calbindin positive neurons were Sp8 positive. TH positive neurons were also usually Sp8 positive, although some were faintly positive. These four types of interneurons were Tbx21 negative. On the other hand large faintly NOS positive external tufted cells were occasionally Tbx21 positive but always Sp8 negative, whereas small NOS positive periglomerular cells without distinctly stained dendrites were usually Sp8 positive and Tbx21 negative. Strangely, most of strongly NOS positive periglomerular cells with distinctly stained dendritic processes were Sp8 negative and Tbx21 negative. Thus Sp8 and Tbx21 immunoreactivities further characterized juxtaglomerular neurons and, especially confirmed the heterogeneity of NOS positive juxtaglomerular neurons.