Analysis of creatinine, vanilmandelic acid, homovanillic acid and uric acid in urine by micellar electrokinetic chromatography

• Published in: Journal of chromatography. B, Biomedical sciences and applications Vol. 693; no. 2; pp. 463 - 467
• Main Authors: Shirao, Mika K, Suzuki, Sumiko, Kobayashi, Jun, Nakazawa, Hiroyuki, Mochizuki, Emiko
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 06.06.1997; Elsevier Science
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Buffers; Catecholamines; Creatinine; Creatinine - urine; Electrophoresis, Capillary; Fundamental and applied biological sciences. Psychology; Homavanillic acid; Homovanillic Acid - urine; Humans; Hydrogen-Ion Concentration; Non peptidic neurotransmitters, polyamines; Other biological molecules; Reproducibility of Results; Sodium Dodecyl Sulfate; Surface-Active Agents; Uric acid; Uric Acid - urine; Vanilmandelic acid; Vanilmandelic Acid - urine; Creatinine; Catecholamines; Homavanillic acid; Vanilmandelic acid; Uric acid; Human; Urine; Biological fluid; Micellar electrokinetic capillary chromatography; Biological marker; Catecholamine; Metabolism; Vanillyl mandelic acid; Biogenic amine; Simultaneous measurement; Quantitative analysis
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(97)00061-3

A simultaneous determination of vanilmandelic acid, homovanillic acid, creatinine and uric acid using capillary electrophoresis was investigated. The optimum conditions of buffer concentration, pH and surfactant concentration were studied, and high resolution was obtained using a 30 m M phosphate buffer (pH 7.0) containing 150 m M sodium dodecyl sulfate. The detection was by UV absorbance at 245 nm and the column was a fused-silica capillary of 67 cm×75 μm I.D.. The determination of these metabolites in human urine was completed within 15 min without any interferences.