Physical and functional interactions between hematopoietic cell-specific ETS transcription factors and homeodomain proteins

• Published in: Leukemia research Vol. 33; no. 3; pp. 483 - 489
• Main Authors: Yamada, Toshiyuki, Shimizu, Takeshi, Sakurai, Takuya, Nanashima, Naoki, Kihara-Negishi, Fumiko, Suzuki, Mitsuhiro, Fan, Yang, Akita, Miki, Oikawa, Tsuneyuki, Tsuchida, Shigeki
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2009
• Subjects: Animals; Cell Line; ETS family of transcription factors; Functional interaction; Gene Expression Regulation; Hematology, Oncology and Palliative Medicine; Hematopoiesis; Hematopoietic Stem Cells; Homeodomain proteins; Homeodomain Proteins - metabolism; Homeodomain Proteins - physiology; Humans; Leukemogenesis; Mice; Physical interaction; Protein Binding - physiology; Proto-Oncogene Proteins c-ets - metabolism; Proto-Oncogene Proteins c-ets - physiology; Functional interaction; Leukemogenesis; Homeodomain proteins; Physical interaction; ETS family of transcription factors; Hematopoiesis
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/j.leukres.2008.07.002

Abstract To examine the possibility that ETS family transcription factors, PU.1, SPI-B, ELF-1, ERG-3, ETS-1 and TEL, and homeodomain proteins, HOXA10, HOXC13, MEIS1 and PBX1B, function cooperatively, we investigated their interactions. In luciferase assays, HOXA10 and HOXC13 augmented the activity of PU.1 and SPI-B while diminishing that of ELF-1 and ERG-3. MEIS1 diminished the activity of ETS-1. No clear effects were observed for other combinations. Immunoprecipitation assays showed protein–protein interactions among the combinations exhibiting functional interactions. A mutation of HOXC13, which abolished binding to ELF-1, also abolished the diminishing effect on ELF-1. The results suggest functional interaction through physical interactions.