Mapping QTLs influencing rice floral morphology using recombinant inbred lines derived from a cross between Oryza sativa L. and Oryza rufipogon Griff

• Published in: Theoretical and applied genetics Vol. 107; no. 2; pp. 218 - 226
• Main Authors: UGA, Y, FUKUTA, Y, CAI, H. W, IWATA, H, OHSAWA, R, MORISHIMA, H, FUJIMURA, T
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.07.2003; Berlin Springer Nature B.V
• Subjects: Biological and medical sciences; Chromosome Mapping; Crosses, Genetic; Flowers - anatomy & histology; Flowers - genetics; Fundamental and applied biological sciences. Psychology; Gene loci; Genetics; Genomics; Inbreeding; Molecular and cellular biology; Oryza - anatomy & histology; Oryza - genetics; Oryza rufipogon; Oryza sativa; Palea; Phenotype; Polymorphism, Restriction Fragment Length; Quantitative Trait Loci; Reproduction - genetics; Mating system; QTL analysis; Wild rice (Oryza rufipogon Griff.); Genetics; Floral trait; Asian cultivated rice (Oryza sativa L.)
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-003-1227-y

To understand the genetic basis of floral traits associated with the mating system in rice, we analyzed pistil, stamen and glume traits using a recombinant inbred line population, derived from a cross between an Asian cultivated rice ( Oryza sativa L.), Pei-kuh, and a wild rice ( Oryza rufipogon Griff.), W1944. Quantitative trait loci (QTLs) affecting floral morphology were detected by composite interval mapping using a linkage map constructed using 147 markers, mostly RFLPs. A total of 7, 4, 14 and 6 QTLs were detected for traits related to pistil, stamen, and size and shape of the glume, respectively. Comparison of 31 QTLs affecting these organs revealed ten QTLs affecting the different organs in four adjacent regions on chromosomes 2, 4, 5 and 10, but most QTLs (68%) were located separately on the whole chromosomes. Although four QTLs for stigma breadth, anther length and thickness of lemma and palea explained more than 25% of the total phenotypic variance, most QTLs (87%) had smaller effects. These results suggest that quantitative variation observed for pistil, stamen and glume traits is controlled by several distinct genes with small effects.