Prevalence and Distribution of Dengue Virus in Aedes aegypti in Yogyakarta City before Deployment of Wolbachia Infected Aedes aegypti

• Published in: International journal of environmental research and public health Vol. 16; no. 10; p. 1742
• Main Authors: Rahayu, Ayu, Saraswati, Utari, Supriyati, Endah, Kumalawati, Dian Aruni, Hermantara, Rio, Rovik, Anwar, Daniwijaya, Edwin Widyanto, Fitriana, Iva, Setyawan, Sigit, Ahmad, Riris Andono, Wardana, Dwi Satria, Indriani, Citra, Utarini, Adi, Tantowijoyo, Warsito, Arguni, Eggi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.05.2019; MDPI
• Subjects: Aedes - microbiology; Aedes aegypti; Animals; Biological Control Agents; Cities; Dengue fever; Dengue Virus - isolation & purification; Disease transmission; Epidemics; Female; Humidity; Indonesia; Infections; Mosquito Vectors; Mosquitoes; Nursing; Population; Population number; Public health; Rainfall; Relative humidity; Seasonal variations; Seasons; Serogroup; Surveillance; Towns; Vector-borne diseases; Viral diseases; Viruses; Wolbachia; Cities; Indonesia; Germany; Yogyakarta City; prevalence; Aedes aegypti; world mosquito program; dengue
• ISSN: 1660-4601; 1661-7827; 1660-4601
• DOI: 10.3390/ijerph16101742

Indonesia is one of the countries where dengue infection is prevalent. In this study we measure the prevalence and distribution of dengue virus (DENV) DENV-infected in Yogyakarta City, Indonesia, during the wet season when high dengue transmission period occurred, as baseline data before implementation of a Wolbachia-infected trial for dengue control. We applied One-Step Multiplex Real Time PCR (RT-PCR) for the type-specific-detection of dengue viruses in field-caught adult mosquitoes. In a prospective field study conducted from December 2015 to May 2016, adult female were caught from selected areas in Yogyakarta City, and then screened by using RT-PCR. During the survey period, 36 (0.12%) mosquitoes from amongst 29,252 female mosquitoes were positive for a DENV type. In total, 22.20% of dengue-positive mosquitoes were DENV-1, 25% were DENV-2, 17% were DENV-3, but none were positive for DENV-4. This study has provided dengue virus infection prevalence in field-caught and its circulating serotype in Yogyakarta City before deployment of Wolbachia-infected .