Transcriptional Regulation of the MHC Class I HLA-A11 Promoter by the Zinc Finger Protein ZFX

• Published in: Nucleic acids research Vol. 24; no. 10; pp. 1928 - 1935
• Main Authors: L'Haridon, Myriam, Paul, Pascale, Xerri, Jean-Guilhem, Dastot, Hélène, Dolliger, Christelle, Schmid, Michel, de Angelis, Nelly, Grollet, Laurence, Sigaux, François, Degos, Laurent, Gazin, Claude
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 15.05.1996
• Subjects: Animals; Base Sequence; Binding Sites; Cell Line; DNA - chemistry; DNA - metabolism; DNA-Binding Proteins - metabolism; DNA-Binding Proteins - pharmacology; Gene Expression Regulation; HeLa Cells; HLA-A Antigens - genetics; HLA-A11 Antigen; Humans; Kruppel-Like Transcription Factors; Leydig Cells - metabolism; Male; Mice; Molecular Sequence Data; Promoter Regions, Genetic; Recombinant Proteins - pharmacology; Transcription Factors; Transcription, Genetic - drug effects; Transfection; Zinc Fingers
• ISSN: 0305-1048; 1362-4962; 1362-4962
• DOI: 10.1093/nar/24.10.1928

Regulation of the human MHC class I HLA-A11 promoter is governed by a complex array of regulatory elements. One of these elements, shown here to be critical for the transcriptional activity of the promoter, was used to screen a λgt11 library and allowed the identification of a cDNA which coded for the zinc finger protein ZFX. ZFX was shown to bind the sequences AGGGCCCCA and AGGCCCCGA, located respectively at positions −271 to −263 and −242 to −234 of the HLA-A11 promoter, with similar affinities through its three C-terminal zinc fingers. ZFX575, a short isoform of ZFX, activates transcription from the HLA-A11 promoter in a Leydig cell line.