Molecular and Morphometric Update on Italian Salicornia (Chenopodiaceae), with a Focus on the Species S. procumbens s. l

• Published in: Plants (Basel) Vol. 12; no. 2; p. 375
• Main Authors: Sciuto, Katia, Wolf, Marion A, Sfriso, Adriano, Brancaleoni, Lisa, Iberite, Mauro, Iamonico, Duilio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.01.2023; MDPI
• Subjects: biometry; Endemic species; endemism; ETS; Haplotypes; Loci; Phylogenetics; Phylogeny; Populations; psbA-trnH; Salicornia; Salicornia L; Spacer; Speciation; Taxonomic revision; United States > US; Italy; biometry; Salicornia L; endemism; ETS; psbA-trnH
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12020375

is a highly taxonomically problematic genus due to the reduced morphological observable characters. Ten Eurasian species are currently recognized: , , , , , , , , , and . In addition, eleven subspecies are accepted, mainly based on their distribution areas. Along the Venetian coasts and in Sardinia, in the past, an endemic species called was recognized, but this name was later synonymized with subsp. . The aim of the present research is investigating different Italian populations by a molecular point of view, using the nuclear ribosomal external transcribed spacer ETS and the plastid A- H intergenic spacer. A particular focus is on the comparison between Venetian (including those occurring in of ) and Sardinian and other Italian populations of this species. The molecular analyses based on the plastid marker highlight that the Italian populations form two well distinct groups. In particular, some of the Venetian ( of ) and all the Sardinian specimens are genetically distinct (=plastid haplotype 1) from the other investigated populations (=plastid haplotype 2). This indicates that the A H haplotype 1 glassworts represent a distinct entity, which we suppose to coincide with the former . Therefore, we suggest to recognize this taxonomic entity at the subspecies rank, as subsp. comb. and stat. nov. However, contrary to the results found with the plastid A H intergenic spacer, the ETS locus does not show a separation into two distinct clades for , probably due to a different evolution of the two loci. Nevertheless, in the ETS phylogenetic reconstruction, the Sardinian specimens (=ribotypes 2 and 3) are placed, together with a Moroccan sample, in a subclade separated from all the other . These results suggest that the Sardinian populations can represent a subspecies/incipient speciation process, probably due to geographic isolation. In the light of this, morphometric analyses (k-means, MANOVA, PCA, DA, and Box-Plot) have been carried out on the Sardinian and Venetian populations to verify if this distinction is detectable also by a morphological point of view. The morphometric analyses highlight the existence of two groups, concerning both the nuclear and plastid trees. Six characters were found to be diagnostic.