Styrylpyrones from Phellinus linteus Mycelia Alleviate Non-Alcoholic Fatty Liver by Modulating Lipid and Glucose Metabolic Homeostasis in High-Fat and High-Fructose Diet-Fed Mice

• Published in: Antioxidants Vol. 11; no. 5; p. 898
• Main Authors: Chiu, Chun-Hung, Chang, Chun-Chao, Lin, Jia-Jing, Chen, Chin-Chu, Chyau, Charng-Cherng, Peng, Robert Y
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.04.2022; MDPI
• Subjects: Acetic acid; Adiponectin; Body weight; Cell culture; centrifugal partition chromatography (CPC); Chromatography; Diabetes; Dietary supplements; Energy metabolism; Ethyl acetate; Fatty liver; Fructose; High fat diet; hispidin; Homeostasis; hypholomine B; Lipogenesis; Liquid culture; Liver diseases; Mycelia; NAFLD; NF-κB protein; Penicillin; Peroxisome proliferator-activated receptors; Phellinus linteus; Polyphenols; SIRT1 protein; Software; Solvents; Sterol regulatory element-binding protein; styrylpyrone polyphenolics; United States > US; Taiwan; NAFLD; styrylpyrone polyphenolics; Phellinus linteus; dyslipidemia; mice; hepatoprotection; hypholomine B; centrifugal partition chromatography (CPC); hispidin
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox11050898

(PL), an edible and medicinal mushroom containing a diversity of styrylpyrone-type polyphenols, has been shown to have a broad spectrum of bioactivities. In this study, the submerged liquid culture in a 1600-L working volume of fermentor was used for the large-scale production of PL mycelia. Whether PL mycelia extract is effective against nonalcoholic fatty liver disease (NAFLD) is still unclear. In the high fat/high fructose diet (HFD)-induced NAFLD C57BL/6 mice study, the dietary supplementation of ethyl acetate fraction from PL mycelia (PL-EA) for four weeks significantly attenuated an increase in body weight, hepatic lipid accumulation and fasting glucose levels. Mechanistically, PL-EA markedly upregulated the α, genes and , downregulated and ; upregulated proteins PPARγ, pAMPK, and PGC-1α, and downregulated SREBP-1 and NF-κB in the liver of HFD-fed mice. Furthermore, the major purified compounds of hispidin and hypholomine B in PL-EA significantly reduced the level of oleic and palmitic acids (O/P)-induced lipid accumulation through the inhibition of up-regulated lipogenesis and the energy-metabolism related genes, and , in the HepG2 cells. Consequently, these findings suggest that the application of PL-EA is deserving of further investigation for treating NAFLD.