P-Lim, a LIM Homeodomain Factor, is Expressed During Pituitary Organ and Cell Commitment and Synergizes with Pit-1

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 92; no. 7; pp. 2720 - 2724
• Main Authors: Bach, Ingolf, Rhodes, Simon J., Pearse, Richard V., Heinzel, Thorsten, Gloss, Bernd, Scully, Kathleen M., Sawchenko, Paul E., Rosenfeld, Michael G.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 28.03.1995; National Acad Sciences; National Academy of Sciences
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cell lines; Cellular biology; Complementary DNA; Developmental biology; DNA; DNA-Binding Proteins - biosynthesis; DNA-Binding Proteins - metabolism; Gene activation; Gene Expression; Gene Library; Genes; Genomic Library; Homeodomain Proteins - biosynthesis; Homeodomain Proteins - metabolism; In Situ Hybridization; LIM-Homeodomain Proteins; Mice; Molecular Sequence Data; Neurons; Phenotypes; Pituitary gland; Pituitary Gland - metabolism; Pituitary Gland, Anterior - metabolism; Pituitary Gland, Posterior - metabolism; Polymerase Chain Reaction; Proteins; Recombinant Fusion Proteins - biosynthesis; Recombinant Proteins - metabolism; Signal detection; Transcription Factor Pit-1; Transcription Factors - metabolism; Transcription, Genetic; Transfection
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.92.7.2720

A pituitary LIM homeodomain factor, P-Lim, is expressed as Rathke's pouch forms and as specific pituitary cell phenotypes are established, suggesting functional roles throughout pituitary development. While selectively expressed in both anterior and intermediate pituitary in mature mice, P-Lim is also transiently expressed in the developing ventral neural cord and brainstem. P-Lim binds to and activates the promoter of the α-glycoprotein subunit gene, a marker of early pituitary development, and synergizes with Pit-1 in transcriptional activation of genes encoding terminal differentiation markers. The LIM domain of P-Lim specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.