Proteomic profile of vitreous in patients with tubercular uveitis

To elucidate disease-specific host protein profile in vitreous fluid of patients with intraocular inflammation due to tubercular uveitis (TBU). Vitreous samples from 13 patients with TBU (group A), 7 with non-TBU (group B) and 9 with no uveitis (group C) were analysed by shotgun proteomics using Liq...

Full description

Saved in:
Bibliographic Details
Published inTuberculosis (Edinburgh, Scotland) Vol. 126; p. 102036
Main Authors Bansal, Reema, Khan, Mohd M., Dasari, Surendra, Verma, Indu, Goodlett, David R., Manes, Nathan P., Nita-Lazar, Aleksandra, Sharma, Surya P., Kumar, Aman, Singh, Nirbhai, Chakraborti, Anuradha, Gupta, Vishali, Dogra, M.R., Ram, Jagat, Gupta, Amod
Format Journal Article
LanguageEnglish
Published Scotland Elsevier Ltd 01.01.2021
Elsevier Science Ltd
Subjects
Adolescent
Adult
Aged
Apoptosis
Biomarkers
Biomarkers - analysis
Carbohydrates
Case-Control Studies
Chromatography, Liquid - methods
Coagulation
Diabetes mellitus
Extrapulmonary tuberculosis
Female
Gluconeogenesis
Glucose
Glucose metabolism
Glycolysis
Humans
Liquid chromatography
Male
Mass spectrometry
Mass spectroscopy
Metabolism
Middle Aged
Molecular modelling
Mycobacterium tuberculosis - isolation & purification
Protein turnover
Proteins
Proteome - analysis
Proteomics
Proteomics - methods
Shotgun proteomics
Shotguns
Signal transduction
Signaling
Tubercular uveitis
Tuberculosis
Tuberculosis, Ocular - diagnosis
Tuberculosis, Ocular - metabolism
Uveitis
Uveitis - diagnosis
Uveitis - metabolism
Uveitis - microbiology
Vitreous
Vitreous Body - chemistry
Vitreous Body - microbiology
Young Adult
Extrapulmonary tuberculosis
Vitreous
Tubercular uveitis
Tuberculosis
Shotgun proteomics
Uveitis
Online AccessGet full text

Cover

More Information
Summary:To elucidate disease-specific host protein profile in vitreous fluid of patients with intraocular inflammation due to tubercular uveitis (TBU). Vitreous samples from 13 patients with TBU (group A), 7 with non-TBU (group B) and 9 with no uveitis (group C) were analysed by shotgun proteomics using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Differentially expressed proteins (DEPs) were subjected to pathway analysis using WEB-based Gene SeT Analysis Toolkit software. Compared to control groups (B + C combined), group A (TBU) displayed 32 (11 upregulated, 21 downregulated) DEPs, which revealed an upregulation of coagulation cascades, complement and classic pathways, and downregulation of metabolism of carbohydrates, gluconeogenesis, glucose metabolism and glycolysis/gluconeogenesis pathways. When compared to group B (non-TBU) alone, TBU displayed 58 DEPs (21 upregulated, 37 downregulated), with an upregulation of apoptosis, KRAS signaling, diabetes pathways, classic pathways, and downregulation of MTORC1 signaling, glycolysis/gluconeogenesis, and glucose metabolism. This differential protein profile provides novel insights into the molecular mechanisms of TBU and a baseline to explore vitreous biomarkers to differentiate TBU from non-TBU, warranting future studies to identify and validate them as a diagnostic tool in TBU. The enriched pathways generate interesting hypotheses and drive further research.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Present Address: Booz Allen Hamilton, McLean, VA, USA.
IV: in charge of overall direction and planning, critical revision of the article.
VG: sample collection, critical revision of the article.
RB, MK, DRG: Designed and performed the experiments.
Visiting Professor, University of Gdansk, Gdansk, Poland, EU
SD: processed the experimental data, performed the analysis, supervised the manuscript and designed the figures and tables.
AC: inputs in manuscript writing.
Author contribution statement
MK, NM, AN-L: sample processing, analysis and interpretation.
MRD, JR: critical revision of the article.
Both authors have contributed equally.
All authors discussed the results and contributed to the final manuscript.
SPS, AK, NS: experimental work.
RB: Writing and editing of the manuscript, conducting the proteomics experiment, sample collection, literature search.
AG: Conception or design of the work, sample collection, critical revision of the article, in charge of overall direction and planning, final approval of the version to be published
SL: in charge of overall direction and planning.
ISSN:1472-9792
1873-281X
DOI:10.1016/j.tube.2020.102036