Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Vol. 104; no. 7; pp. 1495 - 1503
• Main Authors: Massee, Michelle, Chinn, Kathryn, Lei, Jennifer, Lim, Jeremy J., Young, Conan S., Koob, Thomas J.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.10.2016; Wiley Subscription Services, Inc; John Wiley and Sons Inc
• Subjects: Amnion - chemistry; amniotic membrane; Biomedical materials; Cell Proliferation; Chorion - chemistry; Clinical Device‐Related; dermal wound dressing; Desiccation; dHACM; Humans; Materials research; Materials science; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; stem cells; Wound Healing; stem cells; wound healing; amniotic membrane; dHACM; dermal wound dressing
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.33478

Human-derived placental tissues have been shown in randomized clinical trials to be effective for healing chronic wounds, and have also demonstrated the ability to recruit stem cells to the wound site in vitro and in vivo. In this study, PURION(®) Processed dehydrated human amnion/chorion membrane allografts (dHACM, EpiFix(®) , MiMedx Group, Marietta, GA) were evaluated for their ability to alter stem cell activity in vitro. Human bone marrow mesenchymal stem cells (BM-MSCs), adipose derived stem cells (ADSCs), and hematopoietic stem cells (HSCs) were treated with soluble extracts of dHACM tissue, and were evaluated for cellular proliferation, migration, and cytokine secretion. Stem cells were analyzed for cell number by DNA assay after 24 h, closure of an acellular zone using microscopy over 3 days, and soluble cytokine production in the medium of treated stem cells was analyzed after 3 days using a multiplex ELISA array. Treatment with soluble extracts of dHACM tissue stimulated BM-MSCs, ADSCs, and HSCs to proliferate with a significant increase in cell number after 24 h. dHACM treatment accelerated closure of an acellular zone by ADSCs and BM-MSCs after 3 days, compared to basal medium. BM-MSCs, ADSCs, and HSCs also modulated endogenous production of a number of various soluble signals, including regulators of inflammation, mitogenesis, and wound healing. dHACM treatment promoted increased proliferation and migration of ADSCs, BM-MSCs, and HSCs, along with modulation of secreted proteins from those cells. Therefore, dHACM may impact wound healing by amplifying host stem cell populations and modulating their responses in treated wound tissues. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1495-1503, 2016.