Imaging resident and recruited macrophage contribution to Wallerian degeneration

Wallerian degeneration (WD) is a process of autonomous distal degeneration of axons upon injury. Macrophages (MPs) of the peripheral nervous system (PNS) are the main cellular agent controlling this process. Some evidence suggests that resident PNS-MPs along with MPs of hematogenous origin may be in...

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Published inThe Journal of experimental medicine Vol. 217; no. 11
Main Authors Boissonnas, Alexandre, Louboutin, Floriane, Laviron, Marie, Loyher, Pierre-Louis, Reboussin, Elodie, Barthelemy, Sandrine, Réaux-Le Goazigo, Annabelle, Lobsiger, Christian S., Combadière, Béhazine, Mélik Parsadaniantz, Stéphane, Combadière, Christophe
Format Journal Article
LanguageEnglish
Published United States Rockefeller University Press 02.11.2020
Subjects
Animals
Axons - physiology
Brief Definitive Report
Disease Models, Animal
Female
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Human health and pathology
Innate Immunity and Inflammation
Life Sciences
Macrophages - immunology
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Myelin Sheath - physiology
Neuroinflammation
Nonlinear Optical Microscopy
Remyelination - genetics
Sciatic Nerve - diagnostic imaging
Sciatic Nerve - immunology
Sciatic Nerve - injuries
Transcriptome
Wallerian Degeneration - diagnostic imaging
Wallerian Degeneration - immunology
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Summary:Wallerian degeneration (WD) is a process of autonomous distal degeneration of axons upon injury. Macrophages (MPs) of the peripheral nervous system (PNS) are the main cellular agent controlling this process. Some evidence suggests that resident PNS-MPs along with MPs of hematogenous origin may be involved, but whether these two subsets exert distinct functions is unknown. Combining MP-designed fluorescent reporter mice and coherent anti–Stokes Raman scattering (CARS) imaging of the sciatic nerve, we deciphered the spatiotemporal choreography of resident and recently recruited MPs after injury and unveiled distinct functions of these subsets, with recruited MPs being responsible for efficient myelin stripping and clearance and resident MPs being involved in axonal regrowth. This work provides clues to tackle selectively cellular processes involved in neurodegenerative diseases.
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S. Mélik Parsadaniantz and C. Combadière contributed equally to this paper.
Disclosures: The authors declare no competing interests exist.
ISSN:0022-1007
1540-9538
1540-9538
DOI:10.1084/jem.20200471