Exogenous lysozyme influences Clostridium perfringens colonization and intestinal barrier function in broiler chickens

• Published in: Avian pathology Vol. 39; no. 1; pp. 17 - 24
• Main Authors: Liu, Dan, Guo, Yuming, Wang, Zhong, Yuan, Jianmin
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 01.02.2010; Taylor & Francis Ltd
• Subjects: Animal diseases; Animals; Anti-Infective Agents - administration & dosage; Anti-Infective Agents - metabolism; broiler chickens; broiler feeding; Chickens; Clostridium Infections - drug therapy; Clostridium Infections - metabolism; Clostridium Infections - microbiology; Clostridium Infections - pathology; Clostridium Infections - veterinary; Clostridium perfringens; Clostridium perfringens - drug effects; Clostridium perfringens - growth & development; Colonization; Dietary Supplements; duodenum; E coli; enzyme activity; Enzyme kinetics; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - growth & development; Feed conversion; feed supplements; ileum; intestinal absorption; intestinal barrier function; Intestines - drug effects; Intestines - metabolism; Intestines - microbiology; Intestines - pathology; jejunum; Lactobacillus; Lactobacillus - drug effects; Lactobacillus - growth & development; Lesions; liveweight gain; lysozyme; microbial colonization; Muramidase - administration & dosage; Muramidase - metabolism; necrotic enteritis; Poultry; poultry diseases; Poultry Diseases - drug therapy; Poultry Diseases - metabolism; Poultry Diseases - microbiology; Poultry Diseases - pathology; Small intestine; spleen; Spleen - microbiology; Translocation; Weight Gain - drug effects
• ISSN: 0307-9457; 1465-3338; 1465-3338
• DOI: 10.1080/03079450903447404

Necrotic enteritis is a worldwide poultry disease caused by the overgrowth of Clostridium perfringens in the small intestine. An experiment with a 22 factorial design (supplementation with or without 40 mg lysozyme/kg diet for chickens challenged with or without C. perfringens) was conducted to investigate the inhibitory efficacy of exogenous lysozyme against intestinal colonization by C. perfringens in chickens subject to oral inoculation of C. perfringens type A on days 17 to 20. The C. perfringens challenge resulted in significant increase of C. perfringens, Escherichia coli and Lactobacillus populations in the ileum, bacteria translocation to the spleen, the intestinal lesion scores , There was significantly lower intestinal lysozyme activity in the duodenum and jejunum and weight gain during days 14 to 28 of the experiment. The addition of exogenous lysozyme significantly reduced the concentration of C. perfringens in the ileum and the intestinal lesion scores, inhibited the overgrowth of E. coli and Lactobacillus in the ileum and intestinal bacteria translocation to the spleen, and improved intestinal lysozyme activity in the duodenum and the feed conversion ratio of chickens. These findings suggest that exogenous lysozyme could decrease C. perfringens colonization and improve intestinal barrier function and growth performance of chickens.