Embryoid bodies formation from chicken primordial germ cells
Primordial germ cells (PGCs) were demonstrated to be multipotential because of their differentiation ability in all embryonic lineages and because the pluripotential nature of PGCs caters for recent researches on stem cells. PGCs were cultured in suspension to form embryoid bodies (EBs). The charact...
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Published in | Animal cells and systems Vol. 19; no. 3; pp. 168 - 174 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Daejeon
Taylor & Francis
04.05.2015
Taylor & Francis Ltd 한국통합생물학회 |
Subjects | |
Online Access | Get full text |
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Summary: | Primordial germ cells (PGCs) were demonstrated to be multipotential because of their differentiation ability in all embryonic lineages and because the pluripotential nature of PGCs caters for recent researches on stem cells. PGCs were cultured in suspension to form embryoid bodies (EBs). The characterization of PGCs and EBs was assessed by immunofluorescence technique, reverse transcription-polymerase chain reaction (RT-PCR) and paraffin section for Haematoxylin and Eosin (HE) stain. We established a stable chicken PGC line in vitro and prepared masses of EBs from PGCs. We also demonstrated that PGCs expressed stage-specific and stem cell-specific surface makers: SSEA-1, SSEA-3, Oct4, and Sox2. EBs expressed specific genes from three germ layers: gene AFP from entoderm, gene GATA6 from mesoderm, and gene Sox3 from ectoderm. The HE staining illustrated that EBs developed different cell types; relatively larger EBs (440 µm in diameter) were obtained, which contract rhythmically. We concluded that chicken PGCs were suitable for the formation of EBs. Abundant larger size EBs could be prepared in an effective way with our protocol, which could construct a good animal model and provide a useful clinical platform in studying human embryonic development and cell transplantation field. |
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Bibliography: | G704-000140.2015.19.3.009 |
ISSN: | 1976-8354 2151-2485 |
DOI: | 10.1080/19768354.2014.921644 |